Intravital imaging of cardiac function at the single-cell level
Aguirre, Aaron D.
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CitationAguirre, A. D., C. Vinegoni, M. Sebas, and R. Weissleder. 2014. “Intravital Imaging of Cardiac Function at the Single-Cell Level.” Proceedings of the National Academy of Sciences 111 (31): 11257–62. https://doi.org/10.1073/pnas.1401316111.
AbstractKnowledge of cardiomyocyte biology is limited by the lack of methods to interrogate single-cell physiology in vivo. Here we show that contracting myocytes can indeed be imaged with optical microscopy at high temporal and spatial resolution in the beating murine heart, allowing visualization of individual sarcomeres and measurement of the single cardiomyocyte contractile cycle. Collectively, this has been enabled by efficient tissue stabilization, a prospective real-time cardiac gating approach, an image processing algorithm for motion-artifact-free imaging throughout the cardiac cycle, and a fluorescent membrane staining protocol. Quantification of cardiomyocyte contractile function in vivo opens many possibilities for investigating myocardial disease and therapeutic intervention at the cellular level.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384346
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