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dc.contributor.authorAllport, Jennifer R.
dc.contributor.authorWeissleder, Ralph
dc.date.accessioned2019-09-21T03:37:42Z
dc.date.issued2003
dc.identifier.citationAllport, Jennifer R., and Ralph Weissleder. 2003. “Murine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix In Vitro.” Neoplasia 5 (3): 205–17. https://doi.org/10.1016/s1476-5586(03)80053-2.
dc.identifier.issn1476-5586
dc.identifier.issn1522-8002
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384379*
dc.description.abstractThe purpose of the study was to identify characteristics specific to tumor-derived endothelium that may be important in tumor biology, or for the development of targeted therapeutics or imaging agents. Normal C57BI/6 murine heart or lung endothelium, or C57BI/6 murine Lewis lung carcinoma tumor-derived endothelium was isolated from excised tissues using specific antibodies. The endothelium was cultured using either native fibronectin, or the oncofetal form of fibronectin. Cell surface adhesion molecule expression was analyzed by flow cytometry, and the cellular distribution of specific molecules was examined using indirect immunofluorescence staining. Oncofetal fibronectin was critical for maintaining the phenotype of tumor-derived endothelium, which demonstrated an elongated morphology in vitro, with few cell-cell contacts. They expressed high levels of CD31, CD102, and vascular endothelial cadherin, and constitutively expressed CD62E, CD54, and CD106, indicating an "activated" phenotype. Moreover, they expressed significantly greater levels of Sca-1 and Flk-1 than normal murine endothelium. Cellular distribution of CD31, beta-catenin, and CD106 in tumor-derived endothelium was not continuous at cell borders, as observed in cultures of murine heart endothelium. In conclusion, Lewis lung carcinoma-derived tumor endothelium exhibits a specific phenotype in vitro, distinct from normal endothelium, and could be used as an in vitro tool for developing targeted agents.
dc.language.isoen_US
dc.publisherElsevier
dash.licenseMETA_ONLY
dc.titleMurine Lewis Lung Carcinoma-Derived Endothelium Expresses Markers of Endothelial Activation and Requires Tumor-Specific Extracellular Matrix in Vitro
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalNeoplasia (New York, N.Y.)
dash.depositing.authorWeissleder, Ralph::ea07ce19f187d4fab47c56ee97fa5c5a::600
dc.date.available2019-09-21T03:37:42Z
dash.workflow.comments1Science Serial ID 73324
dc.identifier.doi10.1016/S1476-5586(03)80053-2
dash.source.volume5;3
dash.source.page205


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