Show simple item record

dc.contributor.authorKim, Eunha
dc.contributor.authorYang, Katherine
dc.contributor.authorKohler, Rainer
dc.contributor.authorDubach, John
dc.contributor.authorMikula, Hannes
dc.contributor.authorWeissleder, Ralph
dc.date.accessioned2019-09-21T03:38:00Z
dc.date.issued2015
dc.identifier.citationKim, Eunha, Katherine S. Yang, Rainer H. Kohler, John M. Dubach, Hannes Mikula, and Ralph Weissleder. 2015. “Optimized Near-IR Fluorescent Agents for in Vivo Imaging of Btk Expression.” Bioconjugate Chemistry 26 (8): 1513–18. https://doi.org/10.1021/acs.bioconjchem.5b00152.
dc.identifier.issn1043-1802
dc.identifier.issn1520-4812
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384399*
dc.description.abstractBruton's tyrosine kinase (Btk) is intricately involved in anti-apoptotic signaling pathways in cancer and in regulating innate immune response. A number of Btk inhibitors are in development for use in treating B-cell malignancies and certain immunologic diseases. To develop robust companion imaging diagnostics for in vivo use, we set out to explore the effects of red wavelength fluorochrome modifications of two highly potent irreversible Btk inhibitors, Ibrutinib and AVL-292. Surprisingly, we found that subtle chemical differences in the fluorochrome had considerable effects on target localization. Based on iterative designs, we developed a single optimized version with superb in vivo imaging characteristics enabling single cell Btk imaging in vivo. This agent (Ibrutinib-SiR-COOH) is expected to be a valuable chemical tool in deciphering Btk biology in cancer and host cells in vivo.
dc.language.isoen_US
dc.publisherAmerican Chemical Society
dash.licenseOAP
dc.titleOptimized Near-IR fluorescent agents for in vivo imaging of Btk expression
dc.typeJournal Article
dc.description.versionAccepted Manuscript
dc.relation.journalBioconjugate Chemistry
dash.depositing.authorWeissleder, Ralph::ea07ce19f187d4fab47c56ee97fa5c5a::600
dc.date.available2019-09-21T03:38:00Z
dash.workflow.comments1Science Serial ID 15866
dc.identifier.doi10.1021/acs.bioconjchem.5b00152
dash.source.volume26;8
dash.source.page1513


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record