Applications of High-Throughput RNA Interference Screens to Problems in Cell and Developmental Biology
41952 GEN17517.pdf (1.369Mb)
Access StatusFull text of the requested work is not available in DASH at this time ("restricted access"). For more information on restricted deposits, see our FAQ.
MetadataShow full item record
CitationPerrimon, Norbert, and Bernard Mathey-Prevot. 2007. “Applications of High-Throughput RNA Interference Screens to Problems in Cell and Developmental Biology.” Genetics 175 (1): 7–16. https://doi.org/10.1534/genetics.106.069963.
AbstractRNA interference (RNAi) in tissue culture cells has emerged as an excellent methodology for identifying gene functions systematically and in an unbiased manner. Here, we describe how RNAi high-throughput screening (HTS) in Drosophila cells are currently being performed and emphasize the strengths and weaknesses of the approach. Further, to demonstrate the versatility of the technology, we provide examples of the various applications of the method to problems in signal transduction and cell and developmental biology. Finally, we discuss emerging technological advances that will extend RNAi-based screening methods.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384478
- HMS Scholarly Articles