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dc.contributor.authorPerkins, Lizabeth
dc.contributor.authorHolderbaum, Laura
dc.contributor.authorTao, Rong
dc.contributor.authorHu, Yanhui
dc.contributor.authorSopko, Richelle
dc.contributor.authorMcCall, Kim
dc.contributor.authorYang-Zhou, Donghui
dc.contributor.authorFlockhart, Ian
dc.contributor.authorBinari, Richard
dc.contributor.authorShim, Hye-Seok
dc.contributor.authorMiller, Audrey
dc.contributor.authorHousden, Amy
dc.contributor.authorFoos, Marianna
dc.contributor.authorRandkelv, Sakara
dc.contributor.authorKelley, Colleen
dc.contributor.authorNamgyal, Pema
dc.contributor.authorVillalta, Christians
dc.contributor.authorLiu, Lu-Ping
dc.contributor.authorJiang, Xia
dc.contributor.authorHuan-Huan, Qiao
dc.contributor.authorWang, Xia
dc.contributor.authorFujiyama, Asao
dc.contributor.authorToyoda, Atsushi
dc.contributor.authorAyers, Kathleen
dc.contributor.authorBlum, Allison
dc.contributor.authorCzech, Benjamin
dc.contributor.authorNeumuller, Ralph
dc.contributor.authorYan, Dong
dc.contributor.authorCavallaro, Amanda
dc.contributor.authorHibbard, Karen
dc.contributor.authorHall, Don
dc.contributor.authorCooley, Lynn
dc.contributor.authorHannon, Gregory
dc.contributor.authorLehmann, Ruth
dc.contributor.authorParks, Annette
dc.contributor.authorMohr, Stephanie
dc.contributor.authorUeda, Ryu
dc.contributor.authorKondo, Shu
dc.contributor.authorNi, Jian-Quan
dc.contributor.authorPerrimon, Norbert
dc.date.accessioned2019-09-21T09:03:09Z
dc.date.issued2015
dc.identifier.citationPerkins, Lizabeth A., Laura Holderbaum, Rong Tao, Yanhui Hu, Richelle Sopko, Kim McCall, Donghui Yang-Zhou, et al. 2015. “The Transgenic RNAi Project at Harvard Medical School: Resources and Validation.” Genetics 201 (3): 843–52. https://doi.org/10.1534/genetics.115.180208.
dc.identifier.issn0016-6731
dc.identifier.issn1943-2631
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384481*
dc.description.abstractTo facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for RNAi that work in all tissues, generating a genome-scale collection of RNAi stocks with input from the community, distributing the lines as they are generated through existing stock centers, validating as many lines as possible using RT-qPCR and phenotypic analyses, and developing tools and web resources for identifying RNAi lines and retrieving existing information on their quality. With these goals in mind, here we describe in detail the various tools we developed and the status of the collection, which is currently composed of 11,491 lines and covering 71% of Drosophila genes. Data on the characterization of the lines either by RT-qPCR or phenotype is available on a dedicated website, the RNAi Stock Validation and Phenotypes Project (RSVP, http://www.flyrnai.org/RSVP.html), and stocks are available from three stock centers, the Bloomington Drosophila Stock Center (United States), National Institute of Genetics (Japan), and TsingHua Fly Center (China).
dc.language.isoen_US
dc.publisherGenetics Society of America
dash.licenseMETA_ONLY
dc.titleThe Transgenic RNAi Project at Harvard Medical School: Resources and Validation
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalGenetics
dash.depositing.authorPerrimon, Norbert::4e34fce71a186b488b1846b199007bfb::600
dc.date.available2019-09-21T09:03:09Z
dash.workflow.comments1Science Serial ID 41989
dc.identifier.doi10.1534/genetics.115.180208
dash.source.volume201;3
dash.source.page843


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