High-throughput approaches to dissecting MAPK signaling pathways
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CitationFRIEDMAN, A, and N PERRIMON. 2006. “High-Throughput Approaches to Dissecting MAPK Signaling Pathways.” Methods 40 (3): 262–71. https://doi.org/10.1016/j.ymeth.2006.05.002.
AbstractWith the development of genome-wide RNAi libraries, it is now possible to screen for novel components of mitogen-activated protein kinase (MAPK) pathways in cell culture. Although genetic dissection in model organisms and biochemical approaches in mammalian cells have been successful in identifying the core signaling cassettes of these pathways, high-throughput assays can yield unbiased, functional genomic insight into pathway regulation. We describe general high-throughput approaches to assaying MAPK signaling and the receptor tyrosine kinase (RTK)/extracellular signal-regulated kinase (ERK) pathway in particular using a phospho-specific antibody-based readout of pathway activity. We also provide examples of secondary validation screens and methods for managing large datasets for future in vivo functional characterization.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384487
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