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dc.contributor.authorZeidler, Martin P.
dc.contributor.authorTan, Change
dc.contributor.authorBellaiche, Yohanns
dc.contributor.authorCherry, Sara
dc.contributor.authorHäder, Sabine
dc.contributor.authorGayko, Urte
dc.contributor.authorPerrimon, Norbert
dc.date.accessioned2019-09-21T09:03:18Z
dc.date.issued2004
dc.identifier.citationZeidler, Martin P, Change Tan, Yohanns Bellaiche, Sara Cherry, Sabine Häder, Urte Gayko, and Norbert Perrimon. 2004. “Temperature-Sensitive Control of Protein Activity by Conditionally Splicing Inteins.” Nature Biotechnology 22 (7): 871–76. https://doi.org/10.1038/nbt979.
dc.identifier.issn1087-0156
dc.identifier.issn1546-1696
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384490*
dc.description.abstractConditional or temperature-sensitive (TS) alleles represent useful tools with which to investigate gene function. Indeed, much of our understanding of yeast has relied on temperature-sensitive mutations which, when available, also provide important insights into other model systems. However, the rarity of temperature- sensitive alleles and difficulty in identifying them has limited their use. Here we describe a system to generate temperature- sensitive alleles based on conditionally active inteins. We have identified temperature-sensitive splicing variants of the yeast Saccharomyces cerevisiae vacuolar ATPase subunit (VMA) intein inserted within Gal4 and transferred these into Gal80. We show that Gal80-intein(TS) is able to efficiently provide temporal regulation of the Gal4/upstream activation sequence (UAS) system in a temperature- dependent manner in Drosophila melanogaster. Given the minimal host requirements necessary for temperature- sensitive intein splicing, this technique has the potential to allow the generation and use of conditionally active inteins in multiple host proteins and model systems, thereby widening the use of temperature- sensitive alleles for functional protein analysis.
dc.language.isoen_US
dc.publisherNature Research
dash.licenseMETA_ONLY
dc.titleTemperature-sensitive control of protein activity by conditionally splicing inteins
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalNature Biotechnology
dash.depositing.authorPerrimon, Norbert::4e34fce71a186b488b1846b199007bfb::600
dc.date.available2019-09-21T09:03:18Z
dash.workflow.comments1Science Serial ID 68207
dc.identifier.doi10.1038/nbt979
dash.source.volume22;7
dash.source.page871-876


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