High-throughput RNAi screening in cultured cells: a user's guide
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Echeverri, Christophe J.
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CitationEcheverri, Christophe J., and Norbert Perrimon. 2006. “High-Throughput RNAi Screening in Cultured Cells: A User’s Guide.” Nature Reviews Genetics 7 (5): 373–84. https://doi.org/10.1038/nrg1836.
AbstractRNA interference has re-energized the field of functional genomics by enabling genome-scale loss-of-function screens in cultured cells. Looking back on the lessons that have been learned from the first wave of technology developments and applications in this exciting field, we provide both a user's guide for newcomers to the field and a detailed examination of some more complex issues, particularly concerning optimization and quality control, for more advanced users. From a discussion of cell lines, screening paradigms, reagent types and read-out methodologies, we explore in particular the complexities of designing optimal controls and normalization strategies for these challenging but extremely powerful studies.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384497
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