Show simple item record

dc.contributor.authorLenders, C. M.
dc.contributor.authorLiu, S.
dc.contributor.authorWilmore, D. W.
dc.contributor.authorSampson, L.
dc.contributor.authorDougherty, L. W.
dc.contributor.authorSpiegelman, D.
dc.contributor.authorWillett, Walter C.::94559ea206eef8a8844fc5b80654fa5b::600
dc.date.accessioned2019-09-21T16:10:13Z
dc.date.issued2009
dc.identifier.citationLenders, C M, S Liu, D W Wilmore, L Sampson, L W Dougherty, D Spiegelman, and W C Willett. 2009. “Evaluation of a Novel Food Composition Database That Includes Glutamine and Other Amino Acids Derived from Gene Sequencing Data.” European Journal of Clinical Nutrition 63 (12): 1433–39. https://doi.org/10.1038/ejcn.2009.110.
dc.identifier.issn0954-3007
dc.identifier.issn1476-5640
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41384607*
dc.description.abstractObjectives: To determine the content of glutamine in major food proteins.Subjects/Methods: We used a validated 131-food item food frequency questionnaire (FFQ) to identify the foods that contributed the most to protein intake among 70 356 women in the Nurses' Health Study (NHS, 1984). The content of glutamine and other amino acids in foods was calculated based on protein fractions generated from gene sequencing methods (Swiss Institute of Bioinformatics) and compared with data from conventional (USDA) and modified biochemical (Khun) methods. Pearson correlation coefficients were used to compare the participants' dietary intakes of amino acids by sequencing and USDA methods. Results: The glutamine content varied from 0.01 to to 9.49 g/100 g of food and contributed from 1 to to 33% of total protein for all FFQ foods with protein. When comparing the sequencing and Kuhn's methods, the proportion of glutamine in meat was 4.8 vs 4.4%. Among NHS participants, mean glutamine intake was 6.84 (s.d. = 2.19) g/day and correlation coefficients for amino acid between intakes assessed by sequencing and USDA methods ranged from 0.94 to 0.99 for absolute intake, -0.08 to 0.90 after adjusting for 100 g of protein, and 0.88 to 0.99 after adjusting for 1000 kcal. The between-person coefficient of variation of energy-adjusted intake of glutamine was 16%. Conclusions: These data suggest that (1) glutamine content can be estimated from gene sequencing methods and (2) there is a reasonably wide variation in energy-adjusted glutamine intake, allowing for exploration of glutamine consumption and disease. European Journal of Clinical Nutrition (2009) 63, 1433-1439; doi:10.1038/ejcn.2009.110; published online 16 September 2009
dc.language.isoen_US
dc.publisherSpringer Nature
dash.licenseLAA
dc.titleEvaluation of a novel food composition database that includes glutamine and other amino acids derived from gene sequencing data
dc.typeJournal Article
dc.description.versionAccepted Manuscript
dc.relation.journalEuropean Journal of Clinical Nutrition
dash.depositing.authorSpiegelman, Donna::37eeac21962b33e4e46e7aedde542849::600
dc.date.available2019-09-21T16:10:13Z
dash.workflow.comments1Science Serial ID 34340
dc.identifier.doi10.1038/ejcn.2009.110
dash.source.volume63;12
dash.source.page1433


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record