Identification of two inner-membrane proteins required for the transport of lipopolysaccharide to the outer membrane of Escherichia coli
Gronenberg, Luisa S.
Silhavy, Thomas J.
MetadataShow full item record
CitationRuiz, N., L. S. Gronenberg, D. Kahne, and T. J. Silhavy. 2008. “Identification of Two Inner-Membrane Proteins Required for the Transport of Lipopolysaccharide to the Outer Membrane of Escherichia Coli.” Proceedings of the National Academy of Sciences 105 (14): 5537–42. https://doi.org/10.1073/pnas.0801196105.
AbstractThe outer membrane (OM) of most Gram-negative bacteria contains lipopolysaccharide (LIPS) in the outer leaflet. LPS, or endotoxin, is a molecule of important biological activities. In the host, LPS elicits a potent immune response, while in the bacterium, it plays a crucial role by establishing a barrier to limit entry of hydrophobic molecules. Before LPS is assembled at the OM, it must be synthesized at the inner membrane (IM) and transported across the aqueous periplasmic compartment. Much is known about the biosynthesis of LPS but, until recently, little was known about its transport and assembly. We applied a reductionist bioinformatic approach that takes advantage of the small size of the proteome of the Gram-negative endosymbiont Blochmannia floridanus to search for novel factors involved in OM biogenesis. This led to the discovery of two essential Escherichia coli IM proteins of unknown function, YjgP and YjgQ, which are required for the transport of LIPS to the cell surface. We propose that these two proteins, which we have renamed LptF and LptG, respectively, are the missing transmembrane components of the ABC transporter that, together with LptB, functions to extract LIPS from the IM en route to the OM.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461165
- FAS Scholarly Articles