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dc.contributor.authorShemesh, Jonathan
dc.contributor.authorArye, Tom Ben
dc.contributor.authorAvesar, Jonathan
dc.contributor.authorKang, Joo
dc.contributor.authorFine, Amir
dc.contributor.authorSuper, Michael
dc.contributor.authorMeller, Amit
dc.contributor.authorIngber, Donald
dc.contributor.authorLevenberg, Shulamit
dc.date.accessioned2019-10-03T14:38:07Z
dc.date.issued2014
dc.identifier.citationShemesh, J., T. Ben Arye, J. Avesar, J. H. Kang, A. Fine, M. Super, A. Meller, D. E. Ingber, and S. Levenberg. 2014. “Stationary Nanoliter Droplet Array with a Substrate of Choice for Single Adherent/Nonadherent Cell Incubation and Analysis.” Proceedings of the National Academy of Sciences 111 (31): 11293–98. https://doi.org/10.1073/pnas.1404472111.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461177*
dc.description.abstractMicrofluidic water-in-oil droplets that serve as separate, chemically isolated compartments can be applied for single-cell analysis; however, to investigate encapsulated cells effectively over prolonged time periods, an array of droplets must remain stationary on a versatile substrate for optimal cell compatibility. We present here a platform of unique geometry and substrate versatility that generates a stationary nanodroplet array by using wells branching off a main microfluidic channel. These droplets are confined by multiple sides of a nanowell and are in direct contact with a biocompatible substrate of choice. The device is operated by a unique and reversed loading procedure that eliminates the need for fine pressure control or external tubing. Fluorocarbon oil isolates the droplets and provides soluble oxygen for the cells. By using this approach, the metabolic activity of single adherent cells was monitored continuously over time, and the concentration of viable pathogens in blood-derived samples was determined directly by measuring the number of colony-formed droplets. The method is simple to operate, requires a few microliters of reagent volume, is portable, is reusable, and allows for cell retrieval. This technology may be particularly useful for multiplexed assays for which prolonged and simultaneous visual inspection of many isolated single adherent or nonadherent cells is required.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleStationary nanoliter droplet array with a substrate of choice for single adherent/nonadherent cell incubation and analysis
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorIngber, Donald Elliot::577cf2edd94eeff15bcf7e5951504981::600
dc.date.available2019-10-03T14:38:07Z
dash.workflow.comments1Science Serial ID 91225
dc.identifier.doi10.1073/pnas.1404472111
dash.source.volume111;31
dash.source.page11293


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