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dc.contributor.authorOh, Doogie
dc.contributor.authorYu, Che-Hang
dc.contributor.authorNeedleman, Daniel
dc.date.accessioned2019-10-03T14:38:29Z
dc.date.issued2016
dc.identifier.citationOh, Doogie, Che-Hang Yu, and Daniel J. Needleman. 2016. “Spatial Organization of the Ran Pathway by Microtubules in Mitosis.” Proceedings of the National Academy of Sciences 113 (31): 8729–34. https://doi.org/10.1073/pnas.1607498113.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461206*
dc.description.abstractConcentration gradients of soluble proteins are believed to be responsible for control of morphogenesis of subcellular systems, but the mechanisms that generate the spatial organization of these subcellular gradients remain poorly understood. Here, we use a newly developed multipoint fluorescence fluctuation spectroscopy technique to study the ras-related nuclear protein (Ran) pathway, which forms soluble gradients around chromosomes in mitosis and is thought to spatially regulate microtubule behaviors during spindle assembly. We found that the distribution of components of the Ran pathway that influence microtubule behaviors is determined by their interactions with microtubules, resulting in microtubule nucleators being localized by the microtubules whose formation they stimulate. Modeling and perturbation experiments show that this feedback makes the length of the spindle insensitive to the length scale of the Ran gradient, allows the spindle to assemble outside the peak of the Ran gradient, and explains the scaling of the spindle with cell size. Such feedback between soluble signaling pathways and the mechanics of the cytoskeleton may be a general feature of subcellular organization.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleSpatial organization of the Ran pathway by microtubules in mitosis
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorNeedleman, Daniel Joseph::d96a02ce893259d40dd77efe8101c37d::600
dc.date.available2019-10-03T14:38:29Z
dash.workflow.comments1Science Serial ID 92349
dc.identifier.doi10.1073/pnas.1607498113
dash.source.volume113;31
dash.source.page8729


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