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dc.contributor.authorMerkle, Florian
dc.contributor.authorMaroof, Asif
dc.contributor.authorWataya, Takafumi
dc.contributor.authorSasai, Yoshiki
dc.contributor.authorStuder, Lorenz
dc.contributor.authorEggan, Kevin
dc.contributor.authorSchier, Alexander
dc.date.accessioned2019-10-03T14:38:33Z
dc.date.issued2015
dc.identifier.citationMerkle, F. T., A. Maroof, T. Wataya, Y. Sasai, L. Studer, K. Eggan, and A. F. Schier. 2015. “Generation of Neuropeptidergic Hypothalamic Neurons from Human Pluripotent Stem Cells.” Development 142 (4): 633–43. https://doi.org/10.1242/dev.117978.
dc.identifier.issn0950-1991
dc.identifier.issn1477-9129
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461212*
dc.description.abstractHypothalamic neurons orchestrate many essential physiological and behavioral processes via secreted neuropeptides, and are relevant to human diseases such as obesity, narcolepsy and infertility. We report the differentiation of human pluripotent stem cells into many of the major types of neuropeptidergic hypothalamic neurons, including those producing pro-opiolemelanocortin, agouti-related peptide, hypocretin/orexin, melanin-concentrating hormone, oxytocin, arginine vasopressin, corticotropin-releasing hormone (CRH) or thyrotropin-releasing hormone. Hypothalamic neurons can be generated using a 'self-patterning' strategy that yields a broad array of cell types, or via a more reproducible directed differentiation approach. Stem cell-derived human hypothalamic neurons share characteristic morphological properties and gene expression patterns with their counterparts in vivo, and are able to integrate into the mouse brain. These neurons could form the basis of cellular models, chemical screens or cellular therapies to study and treat common human diseases.
dc.language.isoen_US
dc.publisherCompany of Biologists
dash.licenseLAA
dc.titleGeneration of neuropeptidergic hypothalamic neurons from human pluripotent stem cells
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalDevelopment (Cambridge, England)
dash.depositing.authorEggan, Kevin::0f3c57f5dab3376e0b3c0b177b709475::600
dc.date.available2019-10-03T14:38:33Z
dash.workflow.comments1Science Serial ID 28527
dc.identifier.doi10.1242/dev.117978
dash.source.volume142;4
dash.source.page633


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