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dc.contributor.authorRay, Mridula
dc.contributor.authorWiskow, Ole
dc.contributor.authorKing, Matthew
dc.contributor.authorIsmail, Nidha
dc.contributor.authorErgun, Ayla
dc.contributor.authorWang, Yanqun
dc.contributor.authorPlys, Aaron
dc.contributor.authorDavis, Christopher
dc.contributor.authorKathrein, Katie
dc.contributor.authorSadreyev, Ruslan
dc.contributor.authorBorowsky, Mark
dc.contributor.authorEggan, Kevin
dc.contributor.authorZon, Leonard
dc.contributor.authorGalloway, Jenna
dc.contributor.authorKingston, Robert
dc.date.accessioned2019-10-03T14:38:34Z
dc.date.issued2016
dc.identifier.citationRay, Mridula K., Ole Wiskow, Matthew J. King, Nidha Ismail, Ayla Ergun, Yanqun Wang, Aaron J. Plys, et al. 2016. “CAT7 and cat7l Long Non-Coding RNAs Tune Polycomb Repressive Complex 1 Function during Human and Zebrafish Development.” Journal of Biological Chemistry 291 (37): 19558–72. https://doi.org/10.1074/jbc.m116.730853.
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461214*
dc.description.abstractThe essential functions of polycomb repressive complex 1 (PRC1) in development and gene silencing are thought to involve long non-coding RNAs (lncRNAs), but few specific lncRNAs that guide PRC1 activity are known. We screened for lncRNAs, which co-precipitate with PRC1 from chromatin and found candidates that impact polycomb group protein (PcG)-regulated gene expression in vivo. A novel lncRNA from this screen, CAT7, regulates expression and polycomb group binding at the MNX1 locus during early neuronal differentiation. CAT7 contains a unique tandem repeat domain that shares high sequence similarity to a non-syntenic zebrafish analog, cat7l. Defects caused by interference of cat7l RNA during zebrafish embryogenesis were rescued by human CAT7 RNA, enhanced by interference of a PRC1 component, and suppressed by interference of a known PRC1 target gene, demonstrating cat7l genetically interacts with a PRC1. We propose a model whereby PRC1 acts in concert with specific lncRNAs and that CAT7/cat7l represents convergent lncRNAs that independently evolved to tune PRC1 repression at individual loci.
dc.language.isoen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dash.licenseLAA
dc.titleCAT7 and cat7l Long Non-coding RNAs Tune Polycomb Repressive Complex 1 Function during Human and Zebrafish Development
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalThe Journal of Biological Chemistry
dash.depositing.authorEggan, Kevin::0f3c57f5dab3376e0b3c0b177b709475::600
dc.date.available2019-10-03T14:38:34Z
dash.workflow.comments1Science Serial ID 108887
dc.identifier.doi10.1074/jbc.M116.730853
dash.source.volume291;37
dash.source.page19558


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