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dc.contributor.authorNeedleman, Daniel J.
dc.contributor.authorGroen, Aaron
dc.contributor.authorOhi, Ryoma
dc.contributor.authorMaresca, Tom
dc.contributor.authorMirny, Leonid
dc.contributor.authorMitchison, Tim
dc.contributor.authorBloom, Kerry S.
dc.date.accessioned2019-10-03T14:39:02Z
dc.date.issued2010
dc.identifier.citationNeedleman, Daniel J., Aaron Groen, Ryoma Ohi, Tom Maresca, Leonid Mirny, and Tim Mitchison. 2010. “Fast Microtubule Dynamics in Meiotic Spindles Measured by Single Molecule Imaging: Evidence That the Spindle Environment Does Not Stabilize Microtubules.” Edited by Kerry S. Bloom. Molecular Biology of the Cell 21 (2): 323–33. https://doi.org/10.1091/mbc.e09-09-0816.
dc.identifier.issn1059-1524
dc.identifier.issn1939-4586
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41461242*
dc.description.abstractMetaphase spindles are steady-state ensembles of microtubules that turn over rapidly and slide poleward in some systems. Since the discovery of dynamic instability in the mid-1980s, models for spindle morphogenesis have proposed that microtubules are stabilized by the spindle environment. We used single molecule imaging to measure tubulin turnover in spindles, and nonspindle assemblies, in Xenopus laevis egg extracts. We observed many events where tubulin molecules spend only a few seconds in polymer and thus are difficult to reconcile with standard models of polymerization dynamics. Our data can be quantitatively explained by a simple, phenomenological model-with only one adjustable parameter-in which the growing and shrinking of microtubule ends is approximated as a biased random walk. Microtubule turnover kinetics did not vary with position in the spindle and were the same in spindles and nonspindle ensembles nucleated by Tetrahymena pellicles. These results argue that the high density of microtubules in spindles compared with bulk cytoplasm is caused by local enhancement of nucleation and not by local stabilization. It follows that the key to understanding spindle morphogenesis will be to elucidate how nucleation is spatially controlled.
dc.language.isoen_US
dc.publisherAmerican Society for Cell Biology
dash.licenseLAA
dc.titleFast Microtubule Dynamics in Meiotic Spindles Measured by Single Molecule Imaging: Evidence That the Spindle Environment Does Not Stabilize Microtubules
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalMolecular Biology of the Cell
dash.depositing.authorNeedleman, Daniel Joseph::d96a02ce893259d40dd77efe8101c37d::600
dc.date.available2019-10-03T14:39:02Z
dash.workflow.comments1Science Serial ID 64641
dc.identifier.doi10.1091/mbc.E09-09-0816
dash.source.volume21;2


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