dc.contributor.author | Wu, Tao | |
dc.contributor.author | McCandlish, Andrew C. | |
dc.contributor.author | Gronenberg, Luisa S. | |
dc.contributor.author | Chng, Shu-Sin | |
dc.contributor.author | Silhavy, Thomas J. | |
dc.contributor.author | Kahne, Daniel | |
dc.date.accessioned | 2019-10-03T17:40:59Z | |
dc.date.issued | 2006 | |
dc.identifier.citation | Wu, T., A. C. McCandlish, L. S. Gronenberg, S.-S. Chng, T. J. Silhavy, and D. Kahne. 2006. “Identification of a Protein Complex That Assembles Lipopolysaccharide in the Outer Membrane of Escherichia Coli.” Proceedings of the National Academy of Sciences 103 (31): 11754–59. https://doi.org/10.1073/pnas.0604744103. | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 0744-2831 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:41467396 | * |
dc.description.abstract | The outer membrane of most Gram-negative bacteria is made up of LIPS, and in nearly all bacteria that contain LIPS it is essential for the life of the organism. The lipid portion of this molecule, lipid A, also known as endotoxin, is a potent activator of the innate immune response. More than 50 genes are required to synthesize LIPS and assemble it at the cell surface. Enormous progress has been made in elucidating the structure and biosynthesis of LPS, but until recently the cellular components required for its transport from its site of synthesis in the inner membrane to its final cellular location at the cell surface remained elusive. Here we describe the identification of a protein complex that functions to assemble LPS at the surface of the cell. This complex contains two proteins: Imp, already identified as an essential outer-membrane protein implicated in LPS assembly; and another protein, RlpB, heretofore identified only as a rare lipoprotein. We show that RlpB is also essential for cell viability and that the Imp/RlpB complex is responsible for LIPS reaching the outer surface of the outer membrane. | |
dc.language.iso | en_US | |
dc.publisher | National Academy of Sciences | |
dash.license | LAA | |
dc.title | Identification of a protein complex that assembles lipopolysaccharide in the outer membrane of Escherichia coli | |
dc.type | Journal Article | |
dc.description.version | Version of Record | |
dc.relation.journal | Proceedings of the National Academy of Sciences of the United States of America | |
dash.depositing.author | Kahne, Daniel::ce3002fe8ddc7ebb9d39c7483bc36293::600 | |
dc.date.available | 2019-10-03T17:40:59Z | |
dash.workflow.comments | 1Science Serial ID 89930 | |
dc.identifier.doi | 10.1073/pnas.0604744103 | |
dash.source.volume | 103;31 | |
dash.source.page | 11754 | |