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dc.contributor.authorYuan, Yanqiu
dc.contributor.authorBarrett, Dianah
dc.contributor.authorZhang, Yi
dc.contributor.authorKahne, Daniel
dc.contributor.authorSliz, Piotr
dc.contributor.authorWalker, Suzanne
dc.date.accessioned2019-10-03T17:41:00Z
dc.date.issued2007
dc.identifier.citationYuan, Y., D. Barrett, Y. Zhang, D. Kahne, P. Sliz, and S. Walker. 2007. “Crystal Structure of a Peptidoglycan Glycosyltransferase Suggests a Model for Processive Glycan Chain Synthesis.” Proceedings of the National Academy of Sciences 104 (13): 5348–53. https://doi.org/10.1073/pnas.0701160104.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41467397*
dc.description.abstractPeptidoglycan is an essential polymer that forms a protective shell around bacterial cell membranes. Peptidoglycan biosynthesis is the target of many clinically used antibiotics, including the beta-lactams, imipenems, cephalosporins, and glycopeptides. Resistance to these and other antibiotics has prompted interest in an atomic-level understanding of the enzymes that make peptidoglycan. Representative structures have been reported for most of the enzymes in the pathway. Until now, however, there have been no structures of any peptidoglycan glycosyltransferases (also known as transglycosylases), which catalyze formation of the carbohydrate chains of peptidoglycan from disaccharide subunits on the bacterial cell surface. We report here the 2.1 angstrom crystal structure of the peptidoglycan glycosyltransferase (PGT) domain of Aquifex aeolicus PBP1A. The structure has a different fold from all other glycosyltransferase structures reported to date, but it bears some resemblance to lambda-lysozyme, an enzyme that degrades the carbohydrate chains of peptidoglycan. An analysis of the structure, combined with biochemical information showing that these enzymes are processive, suggests a model for glycan chain polymerization.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleCrystal structure of a peptidoglycan glycosyltransferase suggests a model for processive glycan chain synthesis
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorKahne, Daniel::ce3002fe8ddc7ebb9d39c7483bc36293::600
dc.date.available2019-10-03T17:41:00Z
dash.workflow.comments1Science Serial ID 90033
dc.identifier.doi10.1073/pnas.0701160104
dash.source.volume104;13
dash.source.page5348


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