Meiotic recombination-related DNA synthesis and its implications for cross-over and non-cross-over recombinant formation
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Author
Terasawa, Masahiro
Ogawa, Hideyuki
Tsukamoto, Yasumasa
Shinohara, Miki
Shirahige, Katsuhiko
Kleckner, Nancy
Ogawa, Tomoko
Published Version
https://doi.org/10.1073/pnas.0611490104Metadata
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Terasawa, M., H. Ogawa, Y. Tsukamoto, M. Shinohara, K. Shirahige, N. Kleckner, and T. Ogawa. 2007. “Meiotic Recombination-Related DNA Synthesis and Its Implications for Cross-over and Non-Cross-over Recombinant Formation.” Proceedings of the National Academy of Sciences 104 (14): 5965–70. https://doi.org/10.1073/pnas.0611490104.Abstract
Meiotic recombination-related DNA synthesis (MRDS) was analyzed in Saccharomyces cerevisiae by specifically timed incorporation of thymidine analogs into chromosomes. Lengths and positions of incorporation tracts were determined relative to a known recombination hot spot along DNA, as was the timing and localization of incorporation relative to forming and formed synaptonemal complex in spread chromosomes. Distinct patterns could be specifically associated with the majority cross-over and non-cross-over recombination processes. The results obtained provide direct evidence for key aspects of current consensus recombination models, provide information regarding temporal and spatial relationships between non-cross-over formation and the synaptonemal complex, and raise the possibility that removal of RecA homolog Rad51 plays a key role in regulating onset of MRDS. Finally, classical observations on MRDS in Drosophila, mouse, and lily are readily mapped onto the findings presented here, providing further evidence for a broadly conserved meiotic recombination process.Terms of Use
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