Construction and Characterization of a Monomeric Insulin Receptor
Author
Wu, James Jianping
Guidotti, Guido
Published Version
https://doi.org/10.1074/jbc.M200367200Metadata
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Wu, James Jianping, and Guido Guidotti. 2002. “Construction and Characterization of a Monomeric Insulin Receptor.” Journal of Biological Chemistry 277 (31): 27809–17. https://doi.org/10.1074/jbc.m200367200.Abstract
A mutant insulin receptor was constructed by replacing cysteine residues Cys(524), Cys(682), Cys(683), and Cys(685) with serine. The mutant was expressed in COS7 and Chinese hamster ovary cells, did not form covalently linked dimers, and was present at the cell surface. There was half as much insulin binding activity at the cell surface in cells expressing the mutant compared with that in cells expressing the wild type receptor. The intracellular processing of the mutant receptor was affected, since its beta-subunit migrated more slowly than that of the wild type receptor on SDS-PAGE. The mutant was capable of insulin-dependent autophosphorylation and phosphorylation of insulin receptor substrate-1 in vivo and could be cross-linked into receptor dimers when membrane-bound. The amount of insulin-dependent autophosphorylation of the mutant receptor was half that of the wild type receptor. However, after solubilization the monomeric insulin receptor had minimal autophosphorylation activity, and, unlike the naturally occurring monomeric receptor tyrosine kinases, the solubilized monomeric insulin receptor did not dimerize in response to insulin binding as determined by sucrose density gradient centrifugation.Terms of Use
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