Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus aureus PBP2
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CitationBarrett, D., C. Leimkuhler, L. Chen, D. Walker, D. Kahne, and S. Walker. 2005. “Kinetic Characterization of the Glycosyltransferase Module of Staphylococcus Aureus PBP2.” Journal of Bacteriology 187 (6): 2215–17. https://doi.org/10.1128/jb.187.6.2215-2217.2005.
AbstractWe report the heterologous overexpression and purification of Staphylococcus aureus PBP2 and demonstrate efficient glycan polymerization from lipid II in vitro. S. aureus PBP2 is the first purified gram-positive class A penicillin-binding protein to show good transglycosylase activity. This enables further studies on this important class of enzymes.
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