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dc.contributor.authorHumpherys, David
dc.contributor.authorEggan, Kevin
dc.contributor.authorAkutsu, Hidenori
dc.contributor.authorFriedman, Adam
dc.contributor.authorHochedlinger, Konrad
dc.contributor.authorYanagimachi, Ryuzo
dc.contributor.authorLander, Eric S.
dc.contributor.authorGolub, Todd R.
dc.contributor.authorJaenisch, Rudolf
dc.date.accessioned2019-10-03T17:42:25Z
dc.date.issued2002
dc.identifier.citationHumpherys, D., K. Eggan, H. Akutsu, A. Friedman, K. Hochedlinger, R. Yanagimachi, E. S. Lander, T. R. Golub, and R. Jaenisch. 2002. “Abnormal Gene Expression in Cloned Mice Derived from Embryonic Stem Cell and Cumulus Cell Nuclei.” Proceedings of the National Academy of Sciences 99 (20): 12889–94. https://doi.org/10.1073/pnas.192433399.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41467500*
dc.description.abstractTo assess the extent of abnormal gene expression in clones, we assessed global gene expression by microarray analysis on RNA from the placentas and livers of neonatal cloned mice derived by nuclear transfer (NT) from both cultured embryonic stem cells and freshly isolated cumulus cells. Direct comparison of gene expression profiles of more than 10,000 genes showed that for both donor cell types approximate to4% of the expressed genes in the NT placentas differed dramatically in expression levels from those in controls and that the majority of abnormally expressed genes were common to both types of clones. Importantly, however, the expression of a smaller set of genes differed between the embryonic stem cell- and cumulus cell-derived clones. The livers of the cloned mice also showed abnormal gene expression, although to a lesser extent, and with a different set of affected genes, than seen in the placentas. Our results demonstrate frequent abnormal gene expression in clones, in which most expression abnormalities appear common to the NT procedure whereas others appear to reflect the particular donor nucleus.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleAbnormal gene expression in cloned mice derived from embryonic stem cell and cumulus cell nuclei
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorEggan, Kevin::0f3c57f5dab3376e0b3c0b177b709475::600
dc.date.available2019-10-03T17:42:25Z
dash.workflow.comments1Science Serial ID 89358
dc.identifier.doi10.1073/pnas.192433399
dash.source.volume99;20
dash.source.page12889


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