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dc.contributor.authorNilssen, Esben A.
dc.contributor.authorSynnes, Marianne
dc.contributor.authorKleckner, Nancy
dc.contributor.authorGrallert, Beáta
dc.contributor.authorBoye, Erik
dc.date.accessioned2019-10-03T17:42:27Z
dc.date.issued2003
dc.identifier.citationNilssen, E. A., M. Synnes, N. Kleckner, B. Grallert, and E. Boye. 2003. “Intra-G1 Arrest in Response to UV Irradiation in Fission Yeast.” Proceedings of the National Academy of Sciences 100 (19): 10758–63. https://doi.org/10.1073/pnas.1833769100.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41467503*
dc.description.abstractG(1) is a crucial phase of cell growth because the decision to begin another mitotic cycle is made during this period. Occurrence of DNA damage in G(1) poses a particular challenge, because replication of damaged DNA can be deleterious and because no sister chromatid is present to provide a template for recombinational repair. We therefore have studied the response of Schizosaccharomyces pombe cells to UV irradiation in early G(1) phase. We find that irradiation results in delayed progression through G(1), as manifested most critically in the delayed formation of the pre-replication complex. This delay does not have the molecular hallmarks of known checkpoint responses: it is independent of the checkpoint proteins Rad3, Cds1, and Chk1 and does not elicit inhibitory phosphorylation of Cdc2. Irradiated cells eventually progress into S phase and arrest in early S by a rad3- and cds1-dependent mechanism, most likely the intra-S checkpoint. Caffeine alleviates both the intra-G(1)- and intra-S-phase delays. We suggest that intra-G(1) delay may be widely conserved and discuss significance and possible mechanisms.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleIntra-G1 arrest in response to UV irradiation in fission yeast
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorKleckner, Nancy Elizabeth::f5e44fcf9b08dbb871ef3e73ac14b7e5::600
dc.date.available2019-10-03T17:42:27Z
dash.workflow.comments1Science Serial ID 89522
dc.identifier.doi10.1073/pnas.1833769100
dash.source.volume100;19
dash.source.page10758


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