dc.contributor.author | Lu, Richard | |
dc.contributor.author | Vandegraaff, Nick | |
dc.contributor.author | Cherepanov, Peter | |
dc.contributor.author | Engelman, Alan | |
dc.date.accessioned | 2019-10-05T03:27:04Z | |
dc.date.issued | 2005 | |
dc.identifier.citation | Lu, R., N. Vandegraaff, P. Cherepanov, and A. Engelman. 2005. “Lys-34, Dispensable for Integrase Catalysis, Is Required for Preintegration Complex Function and Human Immunodeficiency Virus Type 1 Replication.” Journal of Virology 79 (19): 12584–91. https://doi.org/10.1128/jvi.79.19.12584-12591.2005. | |
dc.identifier.issn | 0022-538X | |
dc.identifier.issn | 1070-6321 | |
dc.identifier.issn | 1098-5514 | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:41482904 | * |
dc.description.abstract | Retroviral integrases (INs) function in the context of preintegration complexes (PICs). Two conserved Lys residues in the N-terminal domain of human immunodeficiency virus type 1 (HIV-1) IN were analyzed here for their roles in integration and virus replication. Whereas HIV-1(K46A) grew like the wild type, HIV-1(K34A) was dead. Yet recombinant INK34A protein functioned in in vitro integration assays, and Vpr-INK34A efficiently transcomplemented the infectivity defect of an IN active site mutant virus in cells. HIV-1(K34A) was therefore similar to a number of previously characterized mutant viruses that failed to replicate despite encoding catalytically competent IN. To directly analyze mutant PIC function, a sensitive PCR-based integration assay was developed. HIV-1(K34A) and related mutants failed to support detectable levels (< 1% of wild type) of integration. We therefore concluded that mutations like K34A disrupted higher-order interactions important for PIC function/maturation compared to the innate catalytic activity of IN enzyme. | |
dc.language.iso | en_US | |
dc.publisher | American Society for Microbiology | |
dash.license | LAA | |
dc.title | Lys-34, Dispensable for Integrase Catalysis, Is Required for Preintegration Complex Function and Human Immunodeficiency Virus Type 1 Replication | |
dc.type | Journal Article | |
dc.description.version | Version of Record | |
dc.relation.journal | Journal of Virology | |
dash.depositing.author | Engelman, Alan N.::d24f388fc75569a343fc8e6e8fe32650::600 | |
dc.date.available | 2019-10-05T03:27:04Z | |
dash.workflow.comments | 1Science Serial ID 62926 | |
dc.identifier.doi | 10.1128/JVI.79.19.12584-12591.2005 | |
dash.source.volume | 79;19 | |
dash.source.page | 12584 | |