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dc.contributor.authorRoig, Joan
dc.contributor.authorMikhailov, Alexei
dc.contributor.authorBelham, Christopher
dc.contributor.authorAvruch, Joseph
dc.date.accessioned2019-10-05T03:27:28Z
dc.date.issued2002
dc.identifier.citationRoig, J. 2002. “Nercc1, a Mammalian NIMA-Family Kinase, Binds the Ran GTPase and Regulates Mitotic Progression.” Genes & Development 16 (13): 1640–58. https://doi.org/10.1101/gad.972202.
dc.identifier.issn0890-9369
dc.identifier.issn1549-5477
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41482935*
dc.description.abstractThe protein kinase NIMA is an indispensable pleiotropic regulator of mitotic progression in Aspergillus. Although several mammalian NIMA-like kinases (Neks) are known, none appears to have the broad importance for mitotic regulation attributed to NIMA. Nercc1 is a new NIMA-like kinase that regulates chromosome alignment and segregation in mitosis. Its NIMA-like catalytic domain is followed by a noncatalytic tail containing seven repeats homologous to those of the Ran GEF, RCC1, a Ser/Thr/Pro-rich segment, and a coiled-coil domain. Nercc1 binds to another NIMA-like kinase, Nek6, and also binds specifically to the Ran GTPase through both its catalytic and its RCC1-like domains, preferring RanGDP in vivo. Nercc1 exists as a homooligomer and can autoactivate in vitro by autophosphorylation. Nercc1 is a cytoplasmic protein that is activated during mitosis and is avidly phosphorylated by active p34(Cdc2). Microinjection of anti-Nercc1 antibodies in prophase results in spindle abnormalities and/or chromosomal misalignment. In Ptk2 cells the outcome is prometaphase arrest or aberrant chromosome segregation and aneuploidy, whereas in CFPAC-1 cells prolonged arrest in prometaphase is the usual response. Nercc1 and its partner Nek6 represent a new signaling pathway that regulates mitotic progression.
dc.language.isoen_US
dc.publisherCold Spring Harbor Laboratory Press
dash.licenseLAA
dc.titleNercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and regulates mitotic progression
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalGenes & Development
dash.depositing.authorAvruch, Joseph::9aac9685759478ee19677d9b77258031::600
dc.date.available2019-10-05T03:27:28Z
dash.workflow.comments1Science Serial ID 41690
dc.identifier.doi10.1101/gad.972202
dash.source.volume16;13
dash.source.page1640


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