Inhibition of Translation by a Short Element in the 5′ Leader of the Herpes Simplex Virus 1 DNA Polymerase Transcript
Bryant, Kevin F.
Coen, Donald M.
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CitationBryant, K. F., and D. M. Coen. 2007. “Inhibition of Translation by a Short Element in the 5’ Leader of the Herpes Simplex Virus 1 DNA Polymerase Transcript.” Journal of Virology 82 (1): 77–85. https://doi.org/10.1128/jvi.01484-07.
AbstractMany viruses regulate gene expression, both globally and specifically, to achieve maximal rates of replication. During herpes simplex virus 1 infection, translation of the DNA polymerase (Pol) catalytic subunit is inefficient relative to other proteins of the same temporal class (D. R. Yager, A. I. Marcy, and D. M. Coen., J. Virol. 64:2217-2225, 1990). To investigate the mechanisms involved in the inefficient translation of Pol and to determine whether this inefficient translation could affect viral replication, we performed a mutagenic analysis of the 5' end of the pol transcript. We found that a short sequence (similar to 55 bases) in the 5' leader of the transcript is both necessary and sufficient to inhibit translation in rabbit reticulocyte lysates and sufficient to inhibit reporter gene translation in transfected cells. RNase structure mapping experiments indicated that the inhibitory element adopts a structure that contains regions of a double-stranded nature, which may interfere with ribosomal loading and/or scanning. Pol accumulated to similar to 2- to 3-fold-higher levels per mRNA in cells infected with a mutant virus containing a deletion of the similar to 55-base inhibitory element than in cells infected with a control virus containing this element. Additionally, the mutant virus replicated less efficiently than the control virus. These results suggest that the inhibitory element regulates Pol translation during infection and that its inhibition of Pol translation is beneficial for viral replication.
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