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dc.contributor.authorStrang, Blair L.
dc.contributor.authorBoulant, Steeve
dc.contributor.authorChang, Lynne
dc.contributor.authorKnipe, David M.
dc.contributor.authorKirchhausen, Tomas
dc.contributor.authorCoen, Donald M.
dc.date.accessioned2019-10-05T03:27:44Z
dc.date.issued2012
dc.identifier.citationStrang, B. L., S. Boulant, L. Chang, D. M. Knipe, T. Kirchhausen, and D. M. Coen. 2011. “Human Cytomegalovirus UL44 Concentrates at the Periphery of Replication Compartments, the Site of Viral DNA Synthesis.” Journal of Virology 86 (4): 2089–95. https://doi.org/10.1128/jvi.06720-11.
dc.identifier.issn0022-538X
dc.identifier.issn1070-6321
dc.identifier.issn1098-5514
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41482958*
dc.description.abstractThe formation of replication compartments, the subnuclear structures in which the viral DNA genome is replicated, is a hallmark of hapesvirus infections. The localization of proteins and viral DNA within human cytomegalovirus replication compartments is not well characterized. Immunofluorescence analysis demonstrated the accumulation of the viral DNA polymerase subunit UL44 at the periphery of replication compartments and the presence of different populations of UL44 in infected cells. In contrast, the viral single-stranded-DNA binding protein UL57 was distributed throughout replication compartments. Using "click chemistry" to detect 5-ethyny1-2'-deoxyuridine (EdU) incorporation into replicating viral DNA and pulse-chase protocols, we found that viral DNA synthesis occurs at the periphery of replication compartments and that replicated viral DNA subsequently localizes to the interior of replication compartments. The interiors of replication compartments also contain regions in which UL44 and EdU-labeled DNA are absent. The treatment of cells with a viral DNA polymerase inhibitor reversibly caused the dispersal of both 1JL44 and EdU-labeled viral DNA from replication compartments, indicating that ongoing viral DNA synthesis is necessary to maintain the organization of replication compartments. Our results reveal a previously unappreciated complexity of the organization of human cytomegalovirus replication compartments.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleHuman Cytomegalovirus UL44 Concentrates at the Periphery of Replication Compartments, the Site of Viral DNA Synthesis
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalJournal of Virology
dash.depositing.authorCoen, Donald Mark::f1d1eb8434c5ee0d3e2fa13c1a313e4d::600
dc.date.available2019-10-05T03:27:44Z
dash.workflow.comments1Science Serial ID 63197
dc.identifier.doi10.1128/JVI.06720-11
dash.source.volume86;4
dash.source.page2089


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