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dc.contributor.authorDel Bianco, Cristina
dc.contributor.authorVedenko, Anastasia
dc.contributor.authorChoi, Sung Hee
dc.contributor.authorBerger, Michael F.
dc.contributor.authorShokri, Leila
dc.contributor.authorBulyk, Martha L.
dc.contributor.authorBlacklow, Stephen C.
dc.contributor.authorBergmann, Andreas
dc.date.accessioned2019-10-05T03:28:26Z
dc.date.issued2010
dc.identifier.citationDel Bianco, Cristina, Anastasia Vedenko, Sung Hee Choi, Michael F. Berger, Leila Shokri, Martha L. Bulyk, and Stephen C. Blacklow. 2010. “Notch and MAML-1 Complexation Do Not Detectably Alter the DNA Binding Specificity of the Transcription Factor CSL.” Edited by Andreas Bergmann. PLoS ONE 5 (11): e15034. https://doi.org/10.1371/journal.pone.0015034.
dc.identifier.issn1932-6203
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483000*
dc.description.abstractBackground: Canonical Notch signaling is initiated when ligand binding induces proteolytic release of the intracellular part of Notch (ICN) from the cell membrane. ICN then travels into the nucleus where it drives the assembly of a transcriptional activation complex containing the DNA-binding transcription factor CSL, ICN, and a specialized co-activator of the Mastermind family. A consensus DNA binding site motif for the CSL protein was previously defined using selection-based methods, but whether subsequent association of Notch and Mastermind-like proteins affects the DNA binding preferences of CSL has not previously been examined. Principal Findings: Here, we utilized protein-binding microarrays (PBMs) to compare the binding site preferences of isolated CSL with the preferred binding sites of CSL when bound to the CSL-binding domains of all four different human Notch receptors. Measurements were taken both in the absence and in the presence of Mastermind-like-1 (MAML1). Our data show no detectable difference in the DNA binding site preferences of CSL before and after loading of Notch and MAML1 proteins. Conclusions: /Significance: These findings support the conclusion that accrual of Notch and MAML1 promote transcriptional activation without dramatically altering the preferred sites of DNA binding, and illustrate the potential of PBMs to analyze the binding site preferences of multiprotein-DNA complexes.
dc.language.isoen_US
dc.publisherPublic Library of Science
dash.licenseLAA
dc.titleNotch and MAML-1 Complexation Do Not Detectably Alter the DNA Binding Specificity of the Transcription Factor CSL
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalPloS One
dash.depositing.authorBulyk, Martha L.::45ae122f2e8ae5175ec5bb093ae1d488::600
dc.date.available2019-10-05T03:28:26Z
dash.workflow.comments1Science Serial ID 83453
dc.identifier.doi10.1371/journal.pone.0015034
dash.source.volume5;11


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