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dc.contributor.authorDunn, Gavin P.
dc.contributor.authorCheung, Hiu Wing
dc.contributor.authorAgarwalla, Pankaj K.
dc.contributor.authorThomas, Sapana
dc.contributor.authorZektser, Yulia
dc.contributor.authorKarst, Alison M.
dc.contributor.authorBoehm, Jesse S.
dc.contributor.authorWeir, Barbara A.
dc.contributor.authorBerlin, Aaron M.
dc.contributor.authorZou, Lihua
dc.contributor.authorGetz, Gad
dc.contributor.authorLiu, Joyce F.
dc.contributor.authorHirsch, Michelle
dc.contributor.authorVazquez, Francisca
dc.contributor.authorRoot, David E.
dc.contributor.authorBeroukhim, Rameen
dc.contributor.authorDrapkin, Ronny
dc.contributor.authorHahn, William C.
dc.date.accessioned2019-10-05T03:28:30Z
dc.date.issued2014
dc.identifier.citationDunn, G. P., H. W. Cheung, P. K. Agarwalla, S. Thomas, Y. Zektser, A. M. Karst, J. S. Boehm, et al. 2014. “In Vivo Multiplexed Interrogation of Amplified Genes Identifies GAB2 as an Ovarian Cancer Oncogene.” Proceedings of the National Academy of Sciences 111 (3): 1102–7. https://doi.org/10.1073/pnas.1311909111.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483005*
dc.description.abstractHigh-grade serous ovarian cancers are characterized by widespread recurrent copy number alterations. Although some regions of copy number change harbor known oncogenes and tumor suppressor genes, the genes targeted by the majority of amplified or deleted regions in ovarian cancer remain undefined. Here we systematically tested amplified genes for their ability to promote tumor formation using an in vivo multiplexed transformation assay. We identified the GRB2-associated binding protein 2 (GAB2) as a recurrently amplified gene that potently transforms immortalized ovarian and fallopian tube secretory epithelial cells. Cancer cell lines overexpressing GAB2 require GAB2 for survival and show evidence of phosphatidylinositol 3-kinase (PI3K) pathway activation, which was required for GAB2-induced transformation. Cell lines overexpressing GAB2 were as sensitive to PI3K inhibition as cell lines harboring mutant PIK3CA. Together, these observations nominate GAB2 as an ovarian cancer oncogene, identify an alternative mechanism to activate PI3K signaling, and underscore the importance of PI3K signaling in this cancer
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleIn vivo multiplexed interrogation of amplified genes identifies GAB2 as an ovarian cancer oncogene
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorBeroukhim, Rameen::c5fb52f8c466a3133522637bebc1675a::600
dc.date.available2019-10-05T03:28:30Z
dash.workflow.comments1Science Serial ID 91167
dc.identifier.doi10.1073/pnas.1311909111
dash.source.volume111;3
dash.source.page1102


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