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dc.contributor.authorJurak, Igor
dc.contributor.authorKramer, Martha F.
dc.contributor.authorMellor, Joseph C.
dc.contributor.authorLint, Alison van
dc.contributor.authorRoth, Frederick P.
dc.contributor.authorKnipe, David M.
dc.contributor.authorCoen, Donald M.
dc.date.accessioned2019-10-05T03:29:01Z
dc.date.issued2010
dc.identifier.citationJurak, I., M. F. Kramer, J. C. Mellor, A. L. van Lint, F. P. Roth, D. M. Knipe, and D. M. Coen. 2010. “Numerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2.” Journal of Virology 84 (9): 4659–72. https://doi.org/10.1128/jvi.02725-09.
dc.identifier.issn0022-538X
dc.identifier.issn1070-6321
dc.identifier.issn1098-5514
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483034*
dc.description.abstractCertain viruses use microRNAs (miRNAs) to regulate the expression of their own genes, host genes, or both. Previous studies have identified a limited number of miRNAs expressed by herpes simplex viruses 1 and 2 (HSV-1 and -2), some of which are conserved between these two viruses. To more comprehensively analyze the miRNAs expressed by HSV-1 or HSV-2 during productive and latent infection, we applied a massively parallel sequencing approach. We were able to identify 16 and 17 miRNAs expressed by HSV-1 and HSV-2, respectively, including all previously known species, and a number of previously unidentified virus-encoded miRNAs. The genomic positions of most miRNAs encoded by these two viruses are within or proximal to the latency-associated transcript region. Nine miRNAs are conserved in position and/or sequence, particularly in the seed region, between these two viruses. Interestingly, we did not detect an HSV-2 miRNA homolog of HSV-1 miR-H1, which is highly expressed during productive infection, but we did detect abundant expression of miR-H6, whose seed region is conserved with HSV-1 miR-H1 and might represent a functional analog. We also identified a highly conserved miRNA family arising from the viral origins of replication. In addition, we detected several pairs of complementary miRNAs and we found miRNA-offset RNAs (moRs) arising from the precursors of HSV-1 and HSV-2 miR-H6 and HSV-2 miR-H4. Our results reveal elements of miRNA conservation and divergence that should aid in identifying miRNA functions.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleNumerous Conserved and Divergent MicroRNAs Expressed by Herpes Simplex Viruses 1 and 2
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalJournal of Virology
dash.depositing.authorCoen, Donald Mark::f1d1eb8434c5ee0d3e2fa13c1a313e4d::600
dc.date.available2019-10-05T03:29:01Z
dash.workflow.comments1Science Serial ID 63121
dc.identifier.doi10.1128/JVI.02725-09
dash.source.volume84;9
dash.source.page4659


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