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dc.contributor.authorLimón, Ana
dc.contributor.authorNakajima, Noriko
dc.contributor.authorLu, Richard
dc.contributor.authorGhory, Hina Z.
dc.contributor.authorEngelman, Alan
dc.date.accessioned2019-10-05T03:29:29Z
dc.date.issued2002
dc.identifier.citationLimon, A., N. Nakajima, R. Lu, H. Z. Ghory, and A. Engelman. 2002. “Wild-Type Levels of Nuclear Localization and Human Immunodeficiency Virus Type 1 Replication in the Absence of the Central DNA Flap.” Journal of Virology 76 (23): 12078–86. https://doi.org/10.1128/jvi.76.23.12078-12086.2002.
dc.identifier.issn0022-538X
dc.identifier.issn1070-6321
dc.identifier.issn1098-5514
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483064*
dc.description.abstractNumerous factors have been implicated in the nuclear localization of retroviral preintegration complexes. Whereas sequences in human immunodeficiency virus type 1 (HIV-1) matrix, Vpr, and integrase proteins were initially reported to function specifically in nondividing cells, other recently identified sequences apparently function in dividing cells as well. One of these, the central DNA flap formed during reverse transcription, is specific to lentiviruses. It was previously reported that flap-negative (F-) HIV-1(LAI) was completely defective for viral spread in the MT-4 T-cell line, yet F- HIV-1 vectors were only 2- to 10-fold defective in various single-round transduction assays. To address these different findings, we analyzed the infectivity and nuclear localization phenotypes of two highly related T-cell-tropic strains, HIV-1(NL4-3) and a derivative of HIV-1(HXBc2) deficient for both Vpr and Net In stark contrast to the previous report, F- derivatives of both strains replicated efficiently in MT-4 cells. F- HIV-1(NL4-3) also spread like wild-type HIV-1(NL4-3) in infected Jurkat and primary T-cell cultures. In contrast, F- HIV-1(HXBc2) was replication defective in primary T cells. Results of real-time quantitative PCR assays, however, indicated that F- HIV-1(HXBc2) entered primary T-cell nuclei as efficiently as its wild-type counterpart. Thus, the F- HIV-1(HXBc2) growth defect did not appear to correlate with defective nuclear import. Consistent with this observation, wild-type nef restored replication to F- HIV-1(HXBc2) in primary T cells. Our results indicate that the central DNA flap does not play a major role in either preintegration complex nuclear import or HIV-1 replication in a variety of cell types.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleWild-Type Levels of Nuclear Localization and Human Immunodeficiency Virus Type 1 Replication in the Absence of the Central DNA Flap
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalJournal of Virology
dash.depositing.authorEngelman, Alan N.::d24f388fc75569a343fc8e6e8fe32650::600
dc.date.available2019-10-05T03:29:29Z
dash.workflow.comments1Science Serial ID 62810
dc.identifier.doi10.1128/JVI.76.23.12078-12086.2002
dash.source.volume76;23
dash.source.page12078


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