Burkholderia spp. Alter Pseudomonas aeruginosa Physiology through Iron Sequestration
Author
Weaver, Valerie B.
Kolter, Roberto
Published Version
https://doi.org/10.1128/JB.186.8.2376-2384.2004Metadata
Show full item recordCitation
Weaver, V. B., and R. Kolter. 2004. “Burkholderia Spp. Alter Pseudomonas Aeruginosa Physiology through Iron Sequestration.” Journal of Bacteriology 186 (8): 2376–84. https://doi.org/10.1128/jb.186.8.2376-2384.2004.Abstract
Pseudomonas aeruginosa and members of the Burkholderia cepacia complex often coexist in both the soil and the lungs of cystic fibrosis patients. To gain an understanding of how these different species affect each other's physiology when coexisting, we performed a screen to identify P. aeruginosa genes that are induced in the presence of Burkholderia. A random gene fusion library was constructed in P. aeruginosa PA14 by using a transposon containing a promoterless lacZ gene. Fusion strains were screened for their ability to be induced in the presence of Burkholderia strains in a cross-streak assay. Three fusion strains were induced specifically by Burkholderia species; all three had transposon insertions in genes known to be iron regulated. One of these fusion strains, containing a transposon insertion in gene PA4467, was used to characterize the inducing activity from Burkholderia. Biochemical and genetic evidence demonstrate that ornibactin, a siderophore produced by nearly all B. cepacia strains, can induce P. aeruginosa PA4467. Significantly, PA4467 is induced early in coculture with an ornibactin-producing but not an ornibactin-deficient B. cepacia strain, indicating that ornibactin can be produced by B. cepacia and detected by P. aeruginosa when the two species coexist.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41483155
Collections
- HMS Scholarly Articles [17878]
Contact administrator regarding this item (to report mistakes or request changes)