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dc.contributor.authorYin, Jun
dc.contributor.authorStraight, Paul D.
dc.contributor.authorMcLoughlin, Shaun M.
dc.contributor.authorZhou, Zhe
dc.contributor.authorLin, Alison J.
dc.contributor.authorGolan, David E.
dc.contributor.authorKelleher, Neil L.
dc.contributor.authorKolter, Roberto
dc.contributor.authorWalsh, Christopher T.
dc.date.accessioned2019-10-05T09:46:42Z
dc.date.issued2005
dc.identifier.citationYin, J., P. D. Straight, S. M. McLoughlin, Z. Zhou, A. J. Lin, D. E. Golan, N. L. Kelleher, R. Kolter, and C. T. Walsh. 2005. “Genetically Encoded Short Peptide Tag for Versatile Protein Labeling by Sfp Phosphopantetheinyl Transferase.” Proceedings of the National Academy of Sciences 102 (44): 15815–20. https://doi.org/10.1073/pnas.0507705102.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483157*
dc.description.abstractAn 11-residue peptide with the sequence DSLEFIASKLA was identified from a genomic library of Bacillus subtilis by phage display as an efficient substrate for Sfp phosphopantetheinyl transferase-catalyzed protein labeling by small molecule-CoA conjugates. We name this peptide the "ybbR tag," because part of its sequence is derived from the ybbR ORF in the B. subtilis genome. The site of Sfp-catalyzed ybbR tag labeling was mapped to the underlined Ser residue, and the ybbR tag was found to have a strong tendency for adopting an a-helical conformation in solution. Here we demonstrate that the ybbR tag can be fused to the N or C termini of target proteins or inserted in a flexible loop in the middle of a target protein for site-specific protein labeling by Sfp. The short size of the ybbR tag and its compatibility with various target proteins, the broad substrate specificity of Sfp for labeling the ybbR tag with small-molecule probes of diverse structures, and the high specificity and efficiency of the labeling reaction make Sfp-catalyzed ybbR tag labeling an attractive tool for expanding protein structural and functional diversities by posttranslational modification.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleGenetically encoded short peptide tag for versatile protein labeling by Sfp phosphopantetheinyl transferase
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorKolter, Roberto::f54296e17449791d262d990800c17d94::600
dc.date.available2019-10-05T09:46:42Z
dash.workflow.comments1Science Serial ID 89784
dc.identifier.doi10.1073/pnas.0507705102
dash.source.volume102;44
dash.source.page15815


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