Show simple item record

dc.contributor.authorGarrity, Sean J.
dc.contributor.authorSivanathan, Viknesh
dc.contributor.authorDong, Jijun
dc.contributor.authorLindquist, Susan
dc.contributor.authorHochschild, Ann
dc.date.accessioned2019-10-05T09:47:04Z
dc.date.issued2010
dc.identifier.citationGarrity, S. J., V. Sivanathan, J. Dong, S. Lindquist, and A. Hochschild. 2010. “Conversion of a Yeast Prion Protein to an Infectious Form in Bacteria.” Proceedings of the National Academy of Sciences 107 (23): 10596–601. https://doi.org/10.1073/pnas.0913280107.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483184*
dc.description.abstractPrions are infectious, self-propagating protein aggregates that have been identified in evolutionarily divergent members of the eukaryotic domain of life. Nevertheless, it is not yet known whether prokaryotes can support the formation of prion aggregates. Here we demonstrate that the yeast prion protein Sup35 can access an infectious conformation in Escherichia coli cells and that formation of this material is greatly stimulated by the presence of a transplanted [PSI(+)] inducibility factor, a distinct prion that is required for Sup35 to undergo spontaneous conversion to the prion form in yeast. Our results establish that the bacterial cytoplasm can support the formation of infectious prion aggregates, providing a heterologous system in which to study prion biology.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleConversion of a yeast prion protein to an infectious form in bacteria
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorHochschild, Ann::baa468d4e7e2501d571fac67380f54c6::600
dc.date.available2019-10-05T09:47:04Z
dash.workflow.comments1Science Serial ID 92016
dc.identifier.doi10.1073/pnas.0913280107
dash.source.volume107;23
dash.source.page10596


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record