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dc.contributor.authorLloyd, Sonja
dc.contributor.authorRitchie, Jennifer
dc.contributor.authorRojas-Lopez, Maricarmen
dc.contributor.authorBlumentritt, Carla
dc.contributor.authorPopov, Vsevolod
dc.contributor.authorGreenwich, Jennifer
dc.contributor.authorWaldor, Matthew
dc.contributor.authorTorres, Alfredo
dc.date.accessioned2019-10-05T16:05:34Z
dc.date.issued2012
dc.identifier.citationLloyd, Sonja J., Jennifer M. Ritchie, Maricarmen Rojas-Lopez, Carla A. Blumentritt, Vsevolod L. Popov, Jennifer L. Greenwich, Matthew K. Waldor, and Alfredo G. Torres. 2012. “A Double, Long Polar Fimbria Mutant of Escherichia Coli O157:H7 Expresses Curli and Exhibits ReducedIn VivoColonization.” Edited by S. M. Payne. Infection and Immunity 80 (3): 914–20. https://doi.org/10.1128/iai.05945-11.
dc.identifier.issn0019-9567
dc.identifier.issn1070-6313
dc.identifier.issn1098-5522
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483527*
dc.description.abstractEscherichia coli O157:H7 causes food and waterborne enteric infections that can result in hemorrhagic colitis and life-threatening hemolytic uremic syndrome. Intimate adherence of the bacteria to intestinal epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhesins, including curli and two operons that encode long polar fimbriae (Lpf). To assess the importance of Lpf for intestinal colonization, we performed competition experiments between E. coli O157:H7 and an isogenic Delta lpfA1 Delta lpfA2 double mutant in the infant rabbit model. The mutant was outcompeted in the ileum, cecum, and midcolon, suggesting that Lpf contributes to intestinal colonization. In contrast, the Delta lpfA1 Delta lpfA2 mutant showed increased adherence to colonic epithelial cells in vitro. Transmission electron microscopy revealed curli-like structures on the surface of the Delta lpfA1 Delta lpfA2 mutant, and the presence of curli was confirmed by Congo red binding, immunogold-labeling electron microscopy, immunoblotting, and quantitative real-time reverse transcription-PCR (qRT-PCR) measuring csgA expression. However, deletion of csgA, which encodes the major curli subunit, does not appear to affect intestinal colonization. In addition to suggesting that Lpf can contribute to EHEC intestinal colonization, our observations indicate that the regulatory pathways governing the expression of Lpf and curli are interdependent.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleA Double, Long Polar Fimbria Mutant of Escherichia coli O157:H7 Expresses Curli and Exhibits Reduced in Vivo Colonization
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalInfection and Immunity
dash.depositing.authorWaldor, Matthew K::d323ffce6ff0937ea2fb987a38019253::600
dc.date.available2019-10-05T16:05:34Z
dash.workflow.comments1Science Serial ID 42659
dc.identifier.doi10.1128/IAI.05945-11
dash.source.volume80;3
dash.source.page914


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