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dc.contributor.authorMateo, Roberto
dc.contributor.authorNagamine, Claude M.
dc.contributor.authorSpagnolo, Jeannie
dc.contributor.authorMéndez, Ernesto
dc.contributor.authorRahe, Michael
dc.contributor.authorGale, Michael, Jr.
dc.contributor.authorYuan, Junying
dc.contributor.authorKirkegaard, Karla
dc.date.accessioned2019-10-05T16:05:39Z
dc.date.issued2013
dc.identifier.citationMateo, Roberto, Claude M. Nagamine, Jeannie Spagnolo, Ernesto Méndez, Michael Rahe, Michael Gale Jr., Junying Yuan, and Karla Kirkegaard. 2012. “Inhibition of Cellular Autophagy Deranges Dengue Virion Maturation.” Journal of Virology 87 (3): 1312–21. https://doi.org/10.1128/jvi.02177-12.
dc.identifier.issn0022-538X
dc.identifier.issn1070-6321
dc.identifier.issn1098-5514
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41483535*
dc.description.abstractAutophagy is an important component of the innate immune response, directly destroying many intracellular pathogens. However, some pathogens, including several RNA viruses, subvert the autophagy pathway, or components of the pathway, to facilitate their replication. In the present study, the effect of inhibiting autophagy on the growth of dengue virus was tested using a novel inhibitor, spautin-1 (specific and potent autophagy inhibitor 1). Inhibition of autophagy by spautin-1 generated heat-sensitive, noninfectious dengue virus particles, revealing a large effect of components of the autophagy pathway on viral maturation. A smaller effect on viral RNA accumulation was also observed. Conversely, stimulation of autophagy resulted in increased viral titers and pathogenicity in the mouse. We conclude that the presence of functional autophagy components facilitates viral RNA replication and, more importantly, is required for infectious dengue virus production. Pharmacological inhibition of host processes is an attractive antiviral strategy to avoid selection of treatment-resistant variants, and inhibitors of autophagy may prove to be valuable therapeutics against dengue virus infection and pathogenesis.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleInhibition of Cellular Autophagy Deranges Dengue Virion Maturation
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalJournal of Virology
dash.depositing.authorYuan, Junying::c7e832b918fc7c2356d6c49fe1a0b135::600
dc.date.available2019-10-05T16:05:39Z
dash.workflow.comments1Science Serial ID 63235
dc.identifier.doi10.1128/JVI.02177-12
dash.source.volume87;3
dash.source.page1312


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