A high-throughput cellulase screening system based on droplet microfluidics
Ung, W. Lloyd
Weitz, David A.
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CitationOstafe, Raluca, Radivoje Prodanovic, W. Lloyd Ung, David A. Weitz, and Rainer Fischer. 2014. “A High-Throughput Cellulase Screening System Based on Droplet Microfluidics.” Biomicrofluidics8 (4): 041102. https://doi.org/10.1063/1.4886771.
AbstractA new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41511278
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