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dc.contributor.authorOstafe, Raluca
dc.contributor.authorProdanovic, Radivoje
dc.contributor.authorUng, W. Lloyd
dc.contributor.authorWeitz, David A.
dc.contributor.authorFischer, Rainer
dc.date.accessioned2019-10-09T13:56:51Z
dc.date.issued2014
dc.identifier.citationOstafe, Raluca, Radivoje Prodanovic, W. Lloyd Ung, David A. Weitz, and Rainer Fischer. 2014. “A High-Throughput Cellulase Screening System Based on Droplet Microfluidics.” Biomicrofluidics8 (4): 041102. https://doi.org/10.1063/1.4886771.
dc.identifier.issn1932-1058
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41511278*
dc.description.abstractA new ultra-high-throughput screening assay for the detection of cellulase activity was developed based on microfluidic sorting. Cellulase activity is detected using a series of coupled enzymes leading to the formation of a fluorescent product that can be detected on a chip. Using this method, we have achieved up to 300-fold enrichments of the active population of cells and greater than 90% purity after just one sorting round. In addition, we proved that we can sort the cellulase-expressing cells from mixtures containing less than 1% active cells.
dc.language.isoen_US
dc.publisherAIP Publishing
dash.licenseLAA
dc.titleA high-throughput cellulase screening system based on droplet microfluidics
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalBiomicrofluidics
dash.depositing.authorWeitz, David A.::4c144b06be6d094b21dbe2dd12dbef76::600
dc.date.available2019-10-09T13:56:51Z
dash.workflow.comments1Science Serial ID 16008
dc.identifier.doi10.1063/1.4886771
dash.source.volume8;4


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