Polo-like kinase is required for the fragmentation of pericentriolar Golgi stacks during mitosis
View/ Open
Author
Sütterlin, Christine
Lin, Chin-Yo
Feng, Yang
Ferris, Douglas K.
Erikson, Raymond L.
Malhotra, Vivek
Published Version
https://doi.org/10.1073/pnas.161283998Metadata
Show full item recordCitation
Sutterlin, C., C.-Y. Lin, Y. Feng, D. K. Ferris, R. L. Erikson, and V. Malhotra. 2001. “Polo-like Kinase Is Required for the Fragmentation of Pericentriolar Golgi Stacks during Mitosis.” Proceedings of the National Academy of Sciences98 (16): 9128–32. https://doi.org/10.1073/pnas.161283998.Abstract
The pericentriolar stacks of Golgi cisternae undergo extensive reorganization during mitosis in mammalian cells. GM130 and GRASP65 (Golgi reassembly stacking protein of 65 kDa) are Golgi-associated proteins that are targets of mitotic kinases, and they have also been implicated in the reorganization of the Golgi structure during cell division. Previous studies have reported that mitogen-activated protein kinase kinase-1 (MEK1} and Cdc2 protein kinases are involved in these dynamic changes in the Golgi structure. More recently, the mitotic polo-like kinase (Plk} has been shown to interact with and phosphorylate GRASP65. Here, we provide evidence that Plk is involved in the mitosis-specific fragmentation of the Golgi apparatus. The addition of kinase-defective Plk or immunodepletion of Plk disrupts the fragmentation process. Furthermore, Golgi fragmentation is inhibited by the addition of either full-length or truncated GRASP65. These findings suggest that phospharylation of GRASP65 by Plk may be a critical event in the reorganization of the Golgi structure during mitosis.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41534407
Collections
- FAS Scholarly Articles [17584]
Contact administrator regarding this item (to report mistakes or request changes)