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dc.contributor.authorSourjik, Victor
dc.contributor.authorBerg, Howard C.
dc.date.accessioned2019-10-11T12:29:16Z
dc.date.issued2002
dc.identifier.citationSourjik, V., and H. C. Berg. 2002. “Binding of the Escherichia Coli Response Regulator CheY to Its Target Measured in Vivo by Fluorescence Resonance Energy Transfer.” Proceedings of the National Academy of Sciences99 (20): 12669–74. https://doi.org/10.1073/pnas.192463199.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41534419*
dc.description.abstractIn Escherichia coli chemotaxis, signaling depends on modulation of the level of phosphorylation of CheY, a small protein that couples receptors and flagellar motors. Working in vivo, we used fluorescence resonance energy transfer (FRET) to measure the interaction of CheYsimilar toP with its target, FIN. Binding of CheYsimilar toP to FIN was found to be much less cooperative than motor switching; however, under the conditions of our experiment, most of the FliM appeared to be in the cytoplasm. We studied signal processing times in the chemotaxis pathway by measuring the changes in CheYsimilar toP binding to FIN on flash release of caged chemoeffectors. Following sudden addition of attractant, the amount of CheYsimilar toP bound to FIN decayed exponentially with a rate constant of about 2 s(-1). Following sudden addition of repellent, FliM occupancy increased with a rate constant of about 20 s-1. Using these data, we were able to construct a simple model for the chemotactic pathway and to estimate values of rate constants for several key reactions.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleBinding of the Escherichia coli response regulator CheY to its target measured in vivo by fluorescence resonance energy transfer
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorBerg, Howard Curtis::246ef0f2a815e6b01ad2b2628664c858::600
dc.date.available2019-10-11T12:29:16Z
dash.workflow.comments1Science Serial ID 89369
dc.identifier.doi10.1073/pnas.192463199
dash.source.volume99;20
dash.source.page12669


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