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dc.contributor.authorDephoure, Noah
dc.contributor.authorZhou, Chunshui
dc.contributor.authorVillén, Judit
dc.contributor.authorBeausoleil, Sean A.
dc.contributor.authorBakalarski, Corey E.
dc.contributor.authorElledge, Stephen J.
dc.contributor.authorGygi, Steven P.
dc.date.accessioned2019-10-12T13:15:55Z
dc.date.issued2008
dc.identifier.citationDephoure, N., C. Zhou, J. Villen, S. A. Beausoleil, C. E. Bakalarski, S. J. Elledge, and S. P. Gygi. 2008. “A Quantitative Atlas of Mitotic Phosphorylation.” Proceedings of the National Academy of Sciences105 (31): 10762–67. doi:10.1073/pnas.0805139105.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41542697*
dc.description.abstractThe eukaryotic cell division cycle is characterized by a sequence of orderly and highly regulated events resulting in the duplication and separation of all cellular material into two newly formed daughter cells. Protein phosphorylation by cyclin-dependent kinases (CDKs) drives this cycle. To gain further insight into how phosphorylation regulates the cell cycle, we sought to identify proteins whose phosphorylation is cell cycle regulated. Using stable isotope labeling along with a two-step strategy for phosphopeptide enrichment and high mass accuracy mass spectrometry, we examined protein phosphorylation in a human cell line arrested in the G(1) and mitotic phases of the cell cycle. We report the identification of > 14,000 different phosphorylation events, more than half of which, to our knowledge, have not been described in the literature, along with relative quantitative data for the majority of these sites. We observed > 1,000 proteins with increased phosphorylation in mitosis including many known cell cycle regulators. The majority of sites on regulated phosphopeptides lie in [S/T]P motifs, the minimum required sequence for CDKs, suggesting that many of the proteins may be CDK substrates. Analysis of non-proline site-containing phosphopeptides identified two unique motifs that suggest there are at least two undiscovered mitotic kinases.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleA quantitative atlas of mitotic phosphorylation
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorElledge, Stephen J.::5156efcdc0cac339028b998336bc7bcb::600
dc.date.available2019-10-12T13:15:55Z
dash.workflow.comments1Science Serial ID 90165
dc.identifier.doi10.1073/pnas.0805139105
dash.source.volume105;31
dash.source.page10762


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