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dc.contributor.authorGhose, Chandrabali
dc.contributor.authorKalsy, Anuj
dc.contributor.authorSheikh, Alaullah
dc.contributor.authorRollenhagen, Julianne
dc.contributor.authorJohn, Manohar
dc.contributor.authorYoung, John
dc.contributor.authorRollins, Sean M.
dc.contributor.authorQadri, Firdausi
dc.contributor.authorCalderwood, Stephen B.
dc.contributor.authorKelly, Ciaran P.
dc.contributor.authorRyan, Edward T.
dc.date.accessioned2019-10-12T13:15:56Z
dc.date.issued2007
dc.identifier.citationGhose, C., A. Kalsy, A. Sheikh, J. Rollenhagen, M. John, J. Young, S. M. Rollins, et al. 2007. “Transcutaneous Immunization with Clostridium Difficile Toxoid A Induces Systemic and Mucosal Immune Responses and Toxin A-Neutralizing Antibodies in Mice.” Infection and Immunity75 (6): 2826–32. doi:10.1128/IAI.00127-07.
dc.identifier.issn0019-9567
dc.identifier.issn1070-6313
dc.identifier.issn1098-5522
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41542698*
dc.description.abstractClostridium difficile is the leading cause of nosocomial infectious diarrhea. C difficile produces two toxins (A and B), and systemic and mucosal anti-toxin A antibodies prevent or limit C difficile-associated diarrhea. To evaluate whether transcutaneous immunization with formalin-treated C difficile toxin A (CDA) induces systemic and mucosal anti-CDA immune responses, we transcutaneously immunized three cohorts of mice with CDA with or without immunoadjuvantative cholera toxin (CT) on days 0, 14, 28, and 42. Mice transcutaneously immunized with CDA and CT developed prominent anti-CDA and anti-CT immunoglobulin G (IgG) and IgA responses in serum and anti-CDA and anti-CT IgA responses in stool. Sera from immunized mice were able to neutralize C. difficile toxin A activity in an in vitro cell culture assay. CDA itself demonstrated adjuvant activity and enhanced both serum and stool anti-CT IgA responses. Our results suggest that transcutaneous immunization with CDA toxoid may be a feasible immunization strategy against C. difficile, an important cause of morbidity and mortality against which current preventative strategies are failing.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleTranscutaneous Immunization with Clostridium difficile Toxoid a Induces Systemic and Mucosal Immune Responses and Toxin A-Neutralizing Antibodies in Mice
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalInfection and Immunity
dash.depositing.authorCalderwood, Stephen Beaven::6fb0a9b5954fa73b317740ceaa5d9604::600
dc.date.available2019-10-12T13:15:56Z
dash.workflow.comments1Science Serial ID 42629
dc.identifier.doi10.1128/IAI.00127-07
dash.source.volume75;6
dash.source.page2826


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