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dc.contributor.authorJohn, Manohar
dc.contributor.authorKudva, Indira T.
dc.contributor.authorGriffin, Robert W.
dc.contributor.authorDodson, Allen W.
dc.contributor.authorMcManus, Bethany
dc.contributor.authorKrastins, Bryan
dc.contributor.authorSarracino, David
dc.contributor.authorProgulske-Fox, Ann
dc.contributor.authorHillman, Jeffrey D.
dc.contributor.authorHandfield, Martin
dc.contributor.authorTarr, Phillip I.
dc.contributor.authorCalderwood, Stephen B.
dc.date.accessioned2019-10-12T13:16:13Z
dc.date.issued2005
dc.identifier.citationJohn, M., I. T. Kudva, R. W. Griffin, A. W. Dodson, B. McManus, B. Krastins, D. Sarracino, et al. 2005. “Use of In Vivo-Induced Antigen Technology for Identification of Escherichia Coli O157:H7 Proteins Expressed during Human Infection.” Infection and Immunity73 (5): 2665–79. doi:10.1128/IAI.73.5.2665-2679.2005.
dc.identifier.issn0019-9567
dc.identifier.issn1070-6313
dc.identifier.issn1098-5522
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41542716*
dc.description.abstractUsing in vivo-induced antigen technology (IVIAT), a modified immunoscreening technique that circumvents the need for animal models, we directly identified immunogenic Escherichia coli O157:H7 (O157) proteins expressed either specifically during human infection but not during growth under standard laboratory conditions or at significantly higher levels in vivo than in vitro. IVIAT identified 223 O157 proteins expressed during human infection, several of which were unique to this study. These in vivo-induced (ivi) proteins, encoded by ivi genes, mapped to the backbone, O islands (OIs), and pO157. Lack of in vitro expression of O157-specific ivi proteins was confirmed by proteomic analysis of a mid-exponential-phase culture of E. coli O157 grown in LB broth. Because ivi proteins are expressed in response to specific cues during infection and might help pathogens adapt to and counter hostile in vivo environments, those identified in this study are potential targets for drug and vaccine development. Also, such proteins may be exploited as markers of O157 infection in stool specimens.
dc.language.isoen_US
dc.publisherAmerican Society for Microbiology
dash.licenseLAA
dc.titleUse of in Vivo-Induced Antigen Technology for Identification of Escherichia coli O157:H7 Proteins Expressed during Human Infection
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalInfection and Immunity
dash.depositing.authorCalderwood, Stephen Beaven::6fb0a9b5954fa73b317740ceaa5d9604::600
dc.date.available2019-10-12T13:16:13Z
dash.workflow.comments1Science Serial ID 43489
dc.identifier.doi10.1128/IAI.73.5.2665-2679.2005
dash.source.volume73;5
dash.source.page2665


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