RMRP Is a Non-Coding RNA Essential for Early Murine Development
View/ Open
Author
Rosenbluh, Joseph
Nijhawan, Deepak
Chen, Zhao
Wong, Kwok-Kin
Masutomi, Kenkichi
Hahn, William C.
Bellusci, Saverio
Published Version
https://doi.org/10.1371/journal.pone.0026270Metadata
Show full item recordCitation
Rosenbluh, Joseph, Deepak Nijhawan, Zhao Chen, Kwok-Kin Wong, Kenkichi Masutomi, and William C. Hahn. 2011. “RMRP Is a Non-Coding RNA Essential for Early Murine Development.” Edited by Saverio Bellusci. PLoS ONE 6 (10): e26270. doi:10.1371/journal.pone.0026270.Abstract
RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:41542775
Collections
- HMS Scholarly Articles [17852]
Contact administrator regarding this item (to report mistakes or request changes)