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dc.contributor.authorAlam, S. Munir
dc.contributor.authorMorelli, Marco
dc.contributor.authorDennison, S. Moses
dc.contributor.authorLiao, Hua-Xin
dc.contributor.authorZhang, Ruijun
dc.contributor.authorXia, Shi-Mao
dc.contributor.authorRits-Volloch, Sophia
dc.contributor.authorSun, Li
dc.contributor.authorHarrison, Stephen C.
dc.contributor.authorHaynes, Barton F.
dc.contributor.authorChen, Bing
dc.date.accessioned2019-10-13T16:03:17Z
dc.date.issued2009
dc.identifier.citationAlam, S. M., M. Morelli, S. M. Dennison, H.-X. Liao, R. Zhang, S.-M. Xia, S. Rits-Volloch, et al. 2009. “Role of HIV Membrane in Neutralization by Two Broadly Neutralizing Antibodies.” Proceedings of the National Academy of Sciences 106 (48): 20234–39. doi:10.1073/pnas.0908713106.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41542791*
dc.description.abstractInduction of effective antibody responses against HIV-1 infection remains an elusive goal for vaccine development. Progress may require in-depth understanding of the molecular mechanisms of neutralization by monoclonal antibodies. We have analyzed the molecular actions of two rare, broadly neutralizing, human monoclonal antibodies, 4E10 and 2F5, which target the transiently exposed epitopes in the membrane proximal external region (MPER) of HIV-1 gp41 envelope during viral entry. Both have long CDR H3 loops with a hydrophobic surface facing away from the peptide epitope. We find that the hydrophobic residues of 4E10 mediate a reversible attachment to the viral membrane and that they are essential for neutralization, but not for interaction with gp41. We propose that these antibodies associate with the viral membrane in a required first step and are thereby poised to capture the transient gp41 fusion intermediate. These results bear directly on strategies for rational design of HIV-1 envelope immunogens.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleRole of HIV membrane in neutralization by two broadly neutralizing antibodies
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorHarrison, Stephen::29356e859c6d3698df98e8184352069e::600
dc.date.available2019-10-13T16:03:17Z
dash.workflow.comments1Science Serial ID 90445
dc.identifier.doi10.1073/pnas.0908713106
dash.source.volume106;48
dash.source.page20234


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