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dc.contributor.authorHuang, I-Chueh
dc.contributor.authorBosch, Berend Jan
dc.contributor.authorLi, Fang
dc.contributor.authorLi, Wenhui
dc.contributor.authorLee, Kyoung Hoa
dc.contributor.authorGhiran, Sorina
dc.contributor.authorVasilieva, Natalya
dc.contributor.authorDermody, Terence S.
dc.contributor.authorHarrison, Stephen C.
dc.contributor.authorDormitzer, Philip R.
dc.contributor.authorFarzan, Michael
dc.contributor.authorRottier, Peter J. M.
dc.contributor.authorChoe, Hyeryun
dc.date.accessioned2019-10-13T16:03:28Z
dc.date.issued2006
dc.identifier.citationHuang, I-Chueh, Berend Jan Bosch, Fang Li, Wenhui Li, Kyoung Hoa Lee, Sorina Ghiran, Natalya Vasilieva, et al. 2006. “SARS Coronavirus, but Not Human Coronavirus NL63, Utilizes Cathepsin L to Infect ACE2-Expressing Cells.” Journal of Biological Chemistry 281 (6): 3198–3203. doi:10.1074/jbc.M508381200.
dc.identifier.issn0021-9258
dc.identifier.issn1083-351X
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41542807*
dc.description.abstractViruses require specific cellular receptors to infect their target cells. Angiotensin-converting enzyme 2 ( ACE2) is a cellular receptor for two divergent coronaviruses, SARS coronavirus (SARS-CoV) and human coronavirus NL63 (HCoV-NL63). In addition to host-cell receptors, lysosomal cysteine proteases are required for productive infection by some viruses. Here we show that SARS-CoV, but not HCoV-NL63, utilizes the enzymatic activity of the cysteine protease cathepsin L to infect ACE2-expressing cells. Inhibitors of cathepsin L blocked infection by SARS-CoV and by a retrovirus pseudotyped with the SARS-CoV spike ( S) protein but not infection by HCoV-NL63 or a retrovirus pseudotyped with the HCoV-NL63 S protein. Expression of exogenous cathepsin L substantially enhanced infection mediated by the SARS-CoV S protein and by filovirus GP proteins but not by the HCoV-NL63 S protein or the vesicular stomatitis virus G protein. Finally, an inhibitor of endosomal acidification had substantially less effect on infection mediated by the HCoV-NL63 S protein than on that mediated by the SARS-CoV S protein. Our data indicate that two coronaviruses that utilize a common receptor nonetheless enter cells through distinct mechanisms.
dc.language.isoen_US
dc.publisherAmerican Society for Biochemistry and Molecular Biology
dash.licenseLAA
dc.titleSARS Coronavirus, but Not Human Coronavirus NL63, Utilizes Cathepsin L to Infect ACE2-expressing Cells
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalThe Journal of Biological Chemistry
dash.depositing.authorHarrison, Stephen::29356e859c6d3698df98e8184352069e::600
dc.date.available2019-10-13T16:03:28Z
dash.workflow.comments1Science Serial ID 106417
dc.identifier.doi10.1074/jbc.M508381200
dash.source.volume281;6
dash.source.page3198-3203


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