dc.contributor.author | Agosto, Melina A. | |
dc.contributor.author | Myers, Kimberly S. | |
dc.contributor.author | Ivanovic, Tijana | |
dc.contributor.author | Nibert, Max L. | |
dc.date.accessioned | 2019-10-14T16:05:35Z | |
dc.date.issued | 2008 | |
dc.identifier.citation | Agosto, M. A., K. S. Myers, T. Ivanovic, and M. L. Nibert. 2008. “A Positive-Feedback Mechanism Promotes Reovirus Particle Conversion to the Intermediate Associated with Membrane Penetration.” Proceedings of the National Academy of Sciences 105 (30): 10571–76. doi:10.1073/pnas.0802039105. | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 0744-2831 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:41543010 | * |
dc.description.abstract | Membrane penetration by reovirus is associated with conversion of a metastable intermediate, the ISVP, to a further-disassembled particle, the ISVP*. Factors that promote this conversion in cells are poorly understood. Here, we report the in vitro characterization of a positive-feedback mechanism for promoting ISVP* conversion. At high particle concentration, conversion approximated second-order kinetics, and products of the reaction operated in trans to promote the conversion of target ISVPs. Pore-forming peptide mu 1N, which is released from particles during conversion, was sufficient for promoting activity. A mutant that does not undergo mu 1N release failed to exhibit second-order conversion kinetics and also failed to promote conversion of wild-type target ISVPs. Susceptibility of target ISVPs to promotion in trans was temperature dependent and correlated with target stability, suggesting that capsid dynamics are required-to expose the interacting epitope. A positive-feed back mechanism of promoting escape from the metastable intermediate has not been reported for other viruses but represents a generalizable device for sensing a confined volume, such as that encountered during cell entry. | |
dc.language.iso | en_US | |
dc.publisher | National Academy of Sciences | |
dash.license | LAA | |
dc.title | A positive-feedback mechanism promotes reovirus particle conversion to the intermediate associated with membrane penetration | |
dc.type | Journal Article | |
dc.description.version | Version of Record | |
dc.relation.journal | Proceedings of the National Academy of Sciences of the United States of America | |
dash.depositing.author | Nibert, Max Lee::46f47695d594f27c61e4749c7ba5b23e::600 | |
dc.date.available | 2019-10-14T16:05:35Z | |
dash.workflow.comments | 1Science Serial ID 90164 | |
dc.identifier.doi | 10.1073/pnas.0802039105 | |
dash.source.volume | 105;30 | |
dash.source.page | 10571 | |