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dc.contributor.authorBansal, Dimple
dc.contributor.authorScholl, Claudia
dc.contributor.authorFröhling, Stefan
dc.contributor.authorMcDowell, Elizabeth
dc.contributor.authorLee, Benjamin H.
dc.contributor.authorDöhner, Konstanze
dc.contributor.authorErnst, Patricia
dc.contributor.authorDavidson, Alan J.
dc.contributor.authorDaley, George Q.
dc.contributor.authorZon, Leonard I.
dc.contributor.authorGilliland, D. Gary
dc.contributor.authorHuntly, Brian P.
dc.date.accessioned2019-10-14T16:31:47Z
dc.date.issued2006
dc.identifier.citationBansal, D., C. Scholl, S. Frohling, E. McDowell, B. H. Lee, K. Dohner, P. Ernst, et al. 2006. “Cdx4 Dysregulates Hox Gene Expression and Generates Acute Myeloid Leukemia Alone and in Cooperation with Meis1a in a Murine Model.” Proceedings of the National Academy of Sciences 103 (45): 16924–29. doi:10.1073/pnas.0604579103.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41543103*
dc.description.abstractHOX genes have emerged as critical effectors of leukelmogenesis, but the mechanisms that regulate their expression in leukemia are not well understood. Recent data suggest that the caudal homeobox transcription factors CDX1, CDX2, and CDX4, developmental regulators of HOX gene expression, may contribute to HOX gene dysregulation in leukemia. We report here that CDX4 is expressed normally in early hernatopoietic progenitors and is expressed aberrantly in approximate to 25% of acute myeloid leukemia (AML) patient samples. Cdx4 regulates Hox gene expression in the adult murine hernatopoietic system and dysregulates Hox genes that are implicated in leukemogenesis. Furthermore, bone marrow progenitors that are retrovirally engineered to express Cdx4 serially replate in methylcellulose cultures, grow in liquid culture, and generate a partially penetrant, long-latency AML in bone marrow transplant recipients. Coexpression of the Hox cofactor Meis1a accelerates the Cdx4 AML phenotype and renders it fully penetrant. Structure-function analysis demonstrates that leukemic transformation requires intact Cdx4 transactivation and DNA-binding domains but not the putative Pbx cofactor interaction motif. Together, these data indicate that Cdx4 regulates Hox gene expression in adult hematopoiesis and may serve as an upstream regulator of Hox gene expression in the induction of acute leukemia. Inasmuch as many human leukemias show dysregulated expression of a spectrum of HOX family members, these collective findings also suggest a central role for CDX4 expression in the genesis of acute leukemia.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleCdx4 dysregulates Hox gene expression and generates acute myeloid leukemia alone and in cooperation with Meis1a in a murine model
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorZon, Leonard::81a6e88fe074c911e300de7359c46d7d::600
dc.date.available2019-10-14T16:31:47Z
dash.workflow.comments1Science Serial ID 89897
dc.identifier.doi10.1073/pnas.0604579103
dash.source.volume103;45
dash.source.page16924


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