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dc.contributor.authorKrüger, Marcus
dc.contributor.authorKratchmarova, Irina
dc.contributor.authorBlagoev, Blagoy
dc.contributor.authorTseng, Yu-Hua
dc.contributor.authorKahn, C. Ronald
dc.contributor.authorMann, Matthias
dc.date.accessioned2019-10-14T16:31:54Z
dc.date.issued2008
dc.identifier.citationKruger, M., I. Kratchmarova, B. Blagoev, Y.-H. Tseng, C. R. Kahn, and M. Mann. 2008. “Dissection of the Insulin Signaling Pathway via Quantitative Phosphoproteomics.” Proceedings of the National Academy of Sciences 105 (7): 2451–56. doi:10.1073/pnas.0711713105.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41543108*
dc.description.abstractThe insulin signaling pathway is of pivotal importance in metabolic diseases, such as diabetes, and in cellular processes, such as aging. Insulin activates a tyrosine phosphorylation cascade that branches to create a complex network affecting multiple biological processes. To understand the full spectrum of the tyrosine phosphorylation cascade, we have defined the tyrosine-phosphoproteome of the insulin signaling pathway, using high resolution mass spectrometry in combination with phosphotyrosine immunoprecipitation and stable isotope labeling by amino acids in cell culture (SILAC) in differentiated brown adipocytes. Of 40 identified insulin-induced effectors, 7 have not previously been described in insulin signaling, including SDR, PKC delta binding protein, LRP-6, and PISP/PDZK11, a potential calcium ATPase binding protein. A proteomic interaction screen with PISP/PDZK11 identified the calcium transporting ATPase SERCA2, supporting a connection to calcium signaling. The combination of quantitative phosphoproteomics with cell culture models provides a powerful strategy to dissect the insulin signaling pathways in intact cells.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleDissection of the insulin signaling pathway via quantitative phosphoproteomics
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorTseng, Yu-Hua::741a0bac83a67d2feba94b076e0434e6::600
dc.date.available2019-10-14T16:31:54Z
dash.workflow.comments1Science Serial ID 91979
dc.identifier.doi10.1073/pnas.0711713105
dash.source.volume105;7
dash.source.page2451


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