dc.contributor.author | Bauer, Benedikt W. | |
dc.contributor.author | Rapoport, Tom A. | |
dc.date.accessioned | 2019-10-14T16:31:59Z | |
dc.date.issued | 2009 | |
dc.identifier.citation | Bauer, B. W., and T. A. Rapoport. 2009. “Mapping Polypeptide Interactions of the SecA ATPase during Translocation.” Proceedings of the National Academy of Sciences 106 (49): 20800–805. doi:10.1073/pnas.0910550106. | |
dc.identifier.issn | 0027-8424 | |
dc.identifier.issn | 0744-2831 | |
dc.identifier.issn | 1091-6490 | |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:41543114 | * |
dc.description.abstract | Many bacterial proteins, including most secretory proteins, are translocated across the plasma membrane by the interplay of the cytoplasmic SecA ATPase and a protein-conducting channel formed by the SecY complex. SecA catalyzes the sequential movement of polypeptide segments through the SecY channel. How SecA interacts with a broad range of polypeptide segments is unclear, but structural data raise the possibility that translocation substrates bind into a "clamp'' of SecA. Here, we have used disulfide bridge cross-linking to test this hypothesis. To analyze polypeptide interactions of SecA during translocation, two cysteines were introduced into a translocation intermediate: one that cross-links to the SecY channel and the other one for cross-linking to a cysteine placed at various positions in SecA. Our results show that a translocating polypeptide is indeed captured inside SecA's clamp and moves in an extended conformation through the clamp into the SecY channel. These results define the polypeptide path during SecA-mediated protein translocation and suggest a mechanism by which ATP hydrolysis by SecA is used to move a polypeptide chain through the SecY channel. | |
dc.language.iso | en_US | |
dc.publisher | National Academy of Sciences | |
dash.license | LAA | |
dc.title | Mapping polypeptide interactions of the SecA ATPase during translocation | |
dc.type | Journal Article | |
dc.description.version | Version of Record | |
dc.relation.journal | Proceedings of the National Academy of Sciences of the United States of America | |
dash.depositing.author | Rapoport, Tom A.::e1aaf2126b0716c7ea13c4b8beb21028::600 | |
dc.date.available | 2019-10-14T16:31:59Z | |
dash.workflow.comments | 1Science Serial ID 90413 | |
dc.identifier.doi | 10.1073/pnas.0910550106 | |
dash.source.volume | 106;49 | |
dash.source.page | 20800 | |