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dc.contributor.authorMavrici, Daniela
dc.contributor.authorMarakalala, Mohlopheni J.
dc.contributor.authorHolton, James M.
dc.contributor.authorPrigozhin, Daniil M.
dc.contributor.authorGee, Christine L.
dc.contributor.authorZhang, Yanjia J.
dc.contributor.authorRubin, Eric J.
dc.contributor.authorAlber, Tom
dc.date.accessioned2019-10-15T18:22:16Z
dc.date.issued2014
dc.identifier.citationMavrici, D., M. J. Marakalala, J. M. Holton, D. M. Prigozhin, C. L. Gee, Y. J. Zhang, E. J. Rubin, and T. Alber. 2014. “Mycobacterium Tuberculosis FtsX Extracellular Domain Activates the Peptidoglycan Hydrolase, RipC.” Proceedings of the National Academy of Sciences 111 (22): 8037–42. https://doi.org/10.1073/pnas.1321812111.
dc.identifier.issn0027-8424
dc.identifier.issn0744-2831
dc.identifier.issn1091-6490
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:41552033*
dc.description.abstractBacterial growth and cell division are coordinated with hydrolysis of the peptidoglycan (PG) layer of the cell wall, but the mechanisms of regulation of extracellular PG hydrolases are not well understood. Here we report the biochemical, structural, and genetic analysis of the Mycobacterium tuberculosis homolog of the transmembrane PG-hydrolase regulator, FtsX. The purified FtsX extracellular domain binds the PG peptidase Rv2190c/RipC N-terminal segment, causing a conformational change that activates the enzyme. Deletion of ftsEX and ripC caused similar phenotypes in Mycobacterium smegmatis, as expected for genes in a single pathway. The crystal structure of the FtsX extracellular domain reveals an unprecedented fold containing two lobes connected by a flexible hinge. Mutations in the hydrophobic cleft between the lobes reduce RipC binding in vitro and inhibit FtsX function in M. smegmatis. These studies suggest how FtsX recognizes RipC and support a model inwhich a conformational change in FtsX links the cell division apparatus with PG hydrolysis.
dc.language.isoen_US
dc.publisherNational Academy of Sciences
dash.licenseLAA
dc.titleMycobacterium tuberculosis FtsX extracellular domain activates the peptidoglycan hydrolase, RipC
dc.typeJournal Article
dc.description.versionVersion of Record
dc.relation.journalProceedings of the National Academy of Sciences of the United States of America
dash.depositing.authorRubin, Eric J.::2ccf3691d766f02c048797e00c06cc44::600
dc.date.available2019-10-15T18:22:16Z
dash.workflow.comments1Science Serial ID 91187
dc.identifier.doi10.1073/pnas.1321812111
dash.source.volume111;22
dash.source.page8037


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